| vMIP对细胞膜上趋化因子受体亲和力的结合特性研究 |
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| 引用本文： | 杨清玲,丁勇兴. vMIP对细胞膜上趋化因子受体亲和力的结合特性研究[J]. 上海交通大学学报(医学版), 2006, 26(4): 389-392 |
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| 作者姓名： | 杨清玲 丁勇兴 |
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| 作者单位： | 蚌埠医学院生化与分子生物学教研室，蚌埠市中心医院肿瘤科 蚌埠233000 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 目的通过病毒巨噬细胞炎性蛋白(vMIP)对外周血中单个核细胞(PBMC)膜上趋化因子受体结合的比较性研究,阐明vMIP与受体的结合能力。方法通过放射性配体受体结合实验,利用饱和实验、动力学实验和特异性分析,鉴定vMIP的受体结合能力。结果vMIP与人、食蟹猴PBMC膜上受体结合的Kd分别为11.1、14.3nmol/L,对趋化因子受体CCR5和CXCR4竞争结合的IC50分别为3.4、4.5nmol/L。结论提示了vMIP与膜上受体有较高的亲和力,并对CCR5和CXCR4具有高度特异性,可能对防治炎症及HIV1感染等具有重要意义。
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| 关 键 词： | 病毒巨噬细胞炎性蛋白 趋化因子受体 放射性配体受体结合实验 |
| 文章编号： | 0258-5898(2006)04-0389-04 |
| 收稿时间： | 2005-05-31 |
| 修稿时间： | 2005-05-31 |
Characteristic Research on the Affinity of vMIP to Combine the Chemokine Receptors on Cell Membrane |
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| YANG Qing-ling,DING Yong-xing. Characteristic Research on the Affinity of vMIP to Combine the Chemokine Receptors on Cell Membrane[J]. Journal of Shanghai Jiaotong University:Medical Science, 2006, 26(4): 389-392 |
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| Authors: | YANG Qing-ling DING Yong-xing |
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| Affiliation: | 1.Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui 233000, China ; 2.Department of Oncology, The Center Hospital of Bengbu |
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| Abstract: | Objective To clarify the combined ability of viral macrophage inflammatory protein (vMIP) to receptors through comparison research of vMIP binding to chemokine receptors of peripheral blood macrophages(PBMCs). Methods Combined ability of vMIP to receptors was measured with radioligand assay and identified with saturation, kinetic and specificity analyses. Results Kd is 11.1 nmol/L to human receptors of PBMC and 14.3 nmol/L to monkey receptors. The IC50 is 3.4 nmol/L to CCR5 and 4.5 nmol/L to CXCR4. Conclusion vMIP has high affinity with receptors and high specificity to CCR5 and CXCR4.This may be of great significance in the prevention and treatment of inflammation and HIV infection. |
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| Keywords: | viral macrophage inflammatory protein chemokine receptor radioligand receptor binding methods |
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