谷氨酰胺对饥饿大鼠肠黏膜免疫系统中CD4~+、CD8~+及SIgA表达的影响

目的:观察谷氨酰胺对饥饿大鼠肠黏膜免疫学指标的影响。方法:以饥饿SD大鼠为模型,将90只雄性SD大鼠随机分成三组(即正常组、饥饿组、谷氨酰胺组)。采用饥饿大鼠模型,运用免疫组织化学染色法标记小肠黏膜中CD4+、CD8+T淋巴细胞及SIgA数量,用图像分析仪统计其含量。结果:与正常组比较,全饥饿组大鼠肠黏膜免疫系统中SIgA及CD4+、CD8+T淋巴细胞数量明显减少,差异有统计学意义(P<0.05)。补充谷氨酰胺(GLN)后,SIgA、CD4+、CD8+T淋巴细胞数量明显升高,补充谷氨酰胺组与全饥饿组比较差异有统计学意义(P<0.01)。补充谷氨酰胺组与正常组比较,SIgA数量在饥饿后3 d、7 d无差异,而在饥饿后5 d、9 d差异有统计学意义(P<0.01);CD8+T淋巴细胞数量在饥饿后各时间点明显减少,差异有统计学意义(P<0.01);CD4+T淋巴细胞数量在饥饿后3 d、5 d未见有明显差异,在饥饿后7 d、9 d则明显减少,差异有统计学意义(P<0.01)。结论:谷氨酰胺可增强饥饿大鼠肠黏膜的免疫功能,发挥肠黏膜的保护作用。

Effect of glutamine on CD4~+,CD8~+ t-lymphocytes and SIgA in intestinal mucosal immune system of complete starved rats

Objective To observe the effect of glutamine on immunological indexes in intestinal mucosal immune system of complete starved rats.Methods 90 male SD rats were randomly divided into 3 groups：normal control,starvation and glutamine.CD4＋,CD8＋T-LYM and SIgA in intestine of rat were labeled by immunohistochemical staining and were measured using imaging analyzer.Results Compared with the normal group,the numbers of CD4＋,CD8＋T-LYM and SIgA in starved rat reduced significantly（P0.05）.After treatment with GLN,the numbers of CD4＋,CD8＋T-LYM and SIgA increased significantly（P0.01）.Compared with the normal group,in the GLN group the numbers of SIgA had significant difference at 5,9 days after starvation（P0.01）and the numbers of CD8＋T-LYM were much lower at all phases after starvation（P0.01）and the numbers of CD4＋T-LYM were much lower at 7,9 days after starvation（P0.01）.Conclusion Glutamine is helpful to increase immune function of intestinal mucosa and can alleviate the damage of intestinal mucosa after starvation.