| 选择性超深低温断血流复苏促进猴脑中神经生长因子和胶质细胞源性神经营养因子的表达 |
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| 引用本文： | Huang XC,Xu W,Jiang JY. 选择性超深低温断血流复苏促进猴脑中神经生长因子和胶质细胞源性神经营养因子的表达[J]. 神经科学通报, 2008, 24(3): 150-154. DOI: 10.1007/s12264-008-1702-1 |
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| 作者姓名： | Huang XC Xu W Jiang JY |
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| 作者单位： | [1]丽水市中心医院神经外科,丽水323000 [2]昆明医学院第二附属医院神经外科,昆明650101 [3]上海交通大学附属仁济医院神经外科,上海200001 |
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| 基金项目： | This work was supported by the Key Program of Natural Science Foundation of Yunnan Province,China (No.2003C0010Z). |
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| 摘 要： | 目的观察常温缺血10min后选择性超深低温断血流复苏后猴脑中神经生长因子（nerve growth factor,NGF）和胶质细胞源性神经营养因子（glial cell line-derived neurotrophicfactor,GDNF）表达的变化。方法等温组及超深低温组实验猴于灌注或复苏死亡后立即开颅取脑,用NGF和GDNF抗体进行免疫组化染色;对额叶恒定视野内NGF和GDNF的阳性细胞记数求阳性率,并统计学分析。结果等温组2只实验猴额叶NGF和GDNF有微量表达,超深低温组4只实验猴额叶NGF和GDNF表达明显上调,与等温组比较差异均极显著俨〈0．01）。结论猴脑选择性超深低温断血流复苏实验可引起NGF和GDNF表达上调,这可能是防止脑缺血的重要保护机制之一。
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| 关 键 词： | 神经生长因子 胶质细胞源性神经营养因子 超深低温断血流 复苏 猴脑 nerve growth factor glial cell line-derived neurotrophicfactor ultraprofound hypothermic circulatory arrest resuscitation monkey brain 选择性 超深低温断血流 复苏 猴脑 神经生长因子 胶质细胞 源性神经营养因子 表达 monkey brain neurotrophic factor glial cell nerve growth factor expressions hypothermia cerebral selective resuscitation mechanism neuron |
| 文章编号： | 1673-7067(2008)03-0150-05 |
| 修稿时间： | 2008-01-17 |
Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey |
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| Xue-Cai Huang,Wei Xu,Ji-Yao Jiang. Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey[J]. Neuroscience Bulletin, 2008, 24(3): 150-154. DOI: 10.1007/s12264-008-1702-1 |
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| Authors: | Xue-Cai Huang Wei Xu Ji-Yao Jiang |
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| Affiliation: | Department of Neurosurgery, Lishui Central Hospital, Lishui, China. |
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| Abstract: | OBJECTIVE: To investigate the expression of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) in monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion. METHODS: The monkeys were immediately removed brain after death in operation of group A (identical temperature perfusion group) and group B (ultraprofound hypothermia perfusion group). Immunohistochemical technique was used to determine frontal cellular expression of NGF and GDNF. Statistics were analyzed by ANOVA analyses with significance level at P < 0.05. RESULTS: The expressions of NGF and GDNF in the group B were significantly higher than those in the group A (P < 0.05). CONCLUSION: NGF and GDNF increased significantly in the monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion. It may be a protective mechanism for neuron survival and neural function recovery. |
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| Keywords: | nerve growth factor glial cell line-derived neurotrophicfactor ultraprofound hypothermic circulatory arrest resuscitation monkey brain |
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