| 小鼠甲状腺球蛋白的提取、纯化和鉴定 |
| |
| 引用本文： | 滕晓春,贾秀坤,范晨玲,王红,郭锐,滕卫平. 小鼠甲状腺球蛋白的提取、纯化和鉴定[J]. 中国现代医学杂志, 2007, 17(12): 1467-1469 |
| |
| 作者姓名： | 滕晓春 贾秀坤 范晨玲 王红 郭锐 滕卫平 |
| |
| 作者单位： | 1. 中国医科大学附属第一医院,内分泌科,辽宁,沈阳,110001
2. 中国医科大学实验技术中心一部,辽宁,沈阳,110001 |
| |
| 摘 要： | 目的 进行小鼠甲状腺球蛋白的纯化和鉴定。方法 小鼠甲状腺组织匀浆后进行盐析和Sphadex G-200凝胶层析2次纯化,纯化后的蛋白质产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和Western免疫印迹鉴定。结果 经纯化的蛋白产物为高纯度的甲状腺球蛋白。结论 Sphadex G-200凝胶层析和Western免疫印迹是进行甲状腺球蛋白纯化和鉴定的一种较好的方法。
|
| 关 键 词： | 小鼠 甲状隙球蛋白 纯化 免疫印迹 |
| 文章编号： | 1005-8982（2007）12-1467-03 |
| 收稿时间： | 2006-11-23 |
| 修稿时间： | 2006-11-23 |
Extraction purification and identification thyroglobulin of mouse |
| |
| TENG Xiao-chun,JIA Xiu-kun,FANG Chen ling,WANG Hong,GUO Rui,TENG Wei-ping. Extraction purification and identification thyroglobulin of mouse[J]. China Journal of Modern Medicine, 2007, 17(12): 1467-1469 |
| |
| Authors: | TENG Xiao-chun JIA Xiu-kun FANG Chen ling WANG Hong GUO Rui TENG Wei-ping |
| |
| Affiliation: | 1.Shenyang 110001, P.R.China; 2.Shenyang 110001, P.R.China |
| |
| Abstract: | [Objective] To purify and identify thyroglobulin of mouse. [Method] Thyroid gland tissues were homogenized and salted out, and then purified by repeated gel filtration on Sphadex G-200. This protein was identified by SDS-PAGE and western Immunoblotting decting. [Result] This protein was verified as the high purified Thyroglobulin. [ Conclusion] Sephadex G-200, together with Immunoblotting is a good way to purify and identify thyroglobulin of mouse. |
| |
| Keywords: | mouse thyroglobulin purification immunoblotting |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
