高效液相色谱法测定人子宫、输卵管及血清中氧氟沙星浓度

目的建立人组织及血清中氧氟沙星含量的HPLC测定方法。方法采用Hypersil C₁₈柱分离,流动相为乙腈-0.01 moL·L^-1磷酸盐缓冲液-0.5 moL·L^-1四丁基溴化铵(9∶91∶4, pH 2.5),流速1.0 mL·min^-1,在UV 294 nm处测定；样品经匀浆或用液氮冷冻研磨至粉末状，用1%曲拉通-100溶解，乙酸乙酯-异丙醇（9∶1）提取，HPLC测定。结果 氧氟沙星浓度在0.2～8.0 μg·mL^-1与峰高比呈线性关系,最小检测限40 ng·mL^-1,用本法对20例妇产科病人进行药物浓度测定,取得了满意的结果。结论此方法适用于人体组织中氧氟沙星浓度的测定，口服后氧氟沙星在人体组织中有良好的分布。

DETERMINATION OF OFLOXACIN IN HUMAN FALLOPIAN TUBE,UTERUS AND SERUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

AIM: To establish a method for determineation of the concentration of ofloxacin in human fallopian tube, uterus and serum. METHODS: The separation was performed on a Spherisob C18 column (Hypersil, 250 mm x 4.6 mm ID, 5 microns) with a mobile phase of acetonitrile-0.01 moL.L-1 potassium dihydrogen phosphate-0.5 mol.L-1 tetrabutylammonium bromide (9:91:4, pH 2.5). The flow rate was 1.0 mL.min-1 and detection was at 294 nm. The samples were homogenated or ground to powder after freezing with liquid nitrogen. 1% triton-100 and certain volume of ethylacetate-isopropanol (10:1) were added, shaken and centrifuged. Then the entire organic layer was transferred to a tube and vacuum dried. The residue was reconstituted in the mobile phase for HPLC. RESULTS: There was a linear relationship between the peak area ratio and the ofloxacin concentration over the range of 0.2-8.0 micrograms.mL-1. The limits of detection was 40 ng.mL-1. Using this method to determine the ofloxacin concentrations in relevant organs as well as in the plasma of patients of the Department of Gynecology, and achieved satisfactary results. CONCLUSION: The method can be applied to assay the ofloxacin concentration in human tissues. Ofloxacin was well distributed in woman fallopian tube, uterus and serum after single oral administration.