中国延边地区朝、汉族男性TNF-α238基因与酒依赖及酒精性肝病的易感性

目的 研究延边地区朝、汉族男性外周血单核细胞( PBMCs)中肿瘤坏死因子-α(TNF-α)-238位点基因型和等位基因频率及其与酒依赖、酒精性肝病(ALD)的关系.方法 采用聚合酶链式反应-限制性片段长度多态(PCR-RFLP)方法检测延边地区朝、汉族男性健康对照组(朝鲜族50名,汉族45名)、无肝病嗜酒组(朝鲜族56名,汉族45名)和酒精性肝病组(朝鲜族58名,汉族53名)中TNF-α-238基因多态性,计算和比较各组基因型及等位基因频率.结果 朝鲜族男性中,TNF-α-238G/A基因型频率在无肝病嗜酒组、酒精性肝病组与健康对照组之间无显著性差异(P＞0.05).未检测出A/A基因型.汉族男性中,TNF-α238G/A基因型频率在无肝病嗜酒组、酒精性肝病组与健康对照组之间无显著性差异(P＞0.05).未检测出A/A基因型.朝鲜族和汉族之间TNF-α-238位点基因型和等位基因频率均无显著性差异(P＞0.05).结论 TNF-α238位点基因多态性在中国延边地区朝、汉族男性中的分布无差异.中国延边地区朝、汉族男性TNF-α基因多态性与酒精依赖及酒精性肝病发生无相关,和国外报道存在明显差异.

Relationship between TNF-α238 gene and alcohol dependence, alcoholic liver disease in Korean and Han men of Yanbian area in China

Objective To investigate the genotype and the allele frequency of tumor necrosis factor-alpha（TNF-α） 238 in peripheral blood mononuclear cells（PBMCs） in Korean and Han men of yanbian area and the relationship between alcohol dependence,alcoholic liver disease（ALD）. Methods Polymerase chain reaction restriction fragment length polymorphisrn（PCR-RFLP） was used to detect TNF-α-238 single nucleotide gene polymorphism in the health control group（ Korean50, Han45 ） ,alcohol dependent group（Korean56, Han45 ） and ALD group（ Korean 58, Han53 ） in Kore- an men and Han men of Yanbian area, calculated and compared the genotype and the allele frequency of three groups. Results In Korean men, the genotype frequency of TNF-α-238G/A had no significant difference between al- cohol dependent group, ALD group and health control group（P〉0.05）. No the genotype of TNF-A/A was detected in all the samples. In Han men, the genotype frequency of TNF-α-238 had no significant difference between alcohol dependent group, ALD group,and health control group（P〉0.05）. No the genotype of TNF-A/A was detected in all the samples. Conclusion There is no difference of TNF-α-238 gene polymorphism＇s distribution in Korean and Han men of Yanbian area. There is no relationship between TNF-α-238 gene polymorphism and alcohol dependence,alco- hol dependent,ALD in Korea and Hart men of Yanbian area with abroad report way existence obvious difference.