| 磷脂酰肌醇激酶的非同位素酶解测定法 |
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| 引用本文： | 张泽林,刘霞,马兰.磷脂酰肌醇激酶的非同位素酶解测定法[J].四川大学学报(医学版),1991(1). |
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| 作者姓名： | 张泽林 刘霞 马兰 |
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| 作者单位： | 药学院生物化学教研室
(张泽林，刘霞)，药学院生物化学教研室(马兰) |
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| 摘 要： | 用磷脂酰肌醇特异性磷脂酶C水解磷脂酰肌醇激酶催化产生的磷脂酰肌醇-4-磷酸,产物1,4-二磷酸肌醇再经碱性磷酸酶水解后,测定磷的含量,建立了磷脂酰肌醇激酶的非同位素酶解测定法。该法回收率可达91％,CV=6.5％。
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| 关 键 词： | 磷脂酰肌醇激酶 活性测定 |
A Nonisotopic Enzymolysis Assay Method of Phosphatidylinositol Kinase |
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| Zhang Zelin,Liu Xia,Ma lan.A Nonisotopic Enzymolysis Assay Method of Phosphatidylinositol Kinase[J].Journal of West China University of Medical Sciences,1991(1). |
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| Authors: | Zhang Zelin Liu Xia Ma lan |
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| Institution: | Zhang Zelin;Liu Xia;Ma lan Department of Biochemistry School of Pharmacy |
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| Abstract: | A nonisotopic enzymolysisassay method of phosphatidylinositol kinase(PIK) has been developed. phosphatidy-linositol 4-phosphate(PIP), phosphorylatdfrom phosphatidylinositol(PI) by PIK washydrolyzed with phosphainositide-specificphospholipase C. The product. inositol 1,4-bisphosphate(IP_2), was separated frominositol 1-phosphate (IP) with Dowex-1column chromatography.Then, the IP_?was hydrolyzed by alkaline phosphataseto yield PI, and the PI was determined bycolorimetry. The PIK activity was definedas PI nmol/mg protein per min. Therecovery was 91%,CV=6.5%. |
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| Keywords: | Phosphatidylinositol kinase Activity assay |
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