颌骨骨肉瘤细胞核形态测量和DNA定量研究

为研究不同组织类型及分化程度的骨肉瘤在细胞核形态及ＤＮＡ含量方面的差异，用多功能图像分析仪对４７例颌骨骨肉瘤进行了细胞核形态测量和ＤＮＡ定量研究。结果发现，随着肿瘤恶性程度的增加，骨母细胞型和软骨母细胞型骨肉瘤的细胞核面积增大，形态更加不规则；纤维母细胞型骨肉瘤的核面积则逐渐变小，细胞核趋于圆形。肿瘤的ＤＮＡ含量和大于５倍体（＞５ｃ）瘤细胞的数量随其恶性程度的增加而增多，近２．５ｃ瘤细胞的数量则逐渐减少。３１例３年随访结果表明，肿瘤ＤＮＡ含量的平均值、大于５ｃ体细胞的数量以及肿瘤的倍体水平是判断颌骨骨肉瘤预后的重要指标。

Image analysis of cell nuclear morphology and DNA content in osteosarcoma of the jaws

The measurement of nuclear morphology and quantitative analysis of nuclear DNA content were made by Interactive Image Analysis System in 47 osteosarcomas of the jaws. The results showed that with a decreased degree of differentiation, the cell nuclei of osteoblastic and chondroblastic osteosarcomas came to be large and irregular, those of fibroblastic osteosarcomas, however, to be small and round. It was revealed that high grade osteosarcomas had increased amounts of DNA and that the mean of DNA content, proportion of hyperploid cells and tumor ploidy were found to be of important prognostic value.