对大鼠他巴唑肾阳虚证动物模型下丘脑-垂体-靶腺轴几项指标的考察

目的:考察他巴唑模型对下丘脑-垂体-肾上腺轴、甲状腺轴、性腺轴的影响,比较性别对他巴唑模型的影响。方法:采用他巴唑模型最为常用的造模时间(15天)分成3个剂量组进行造模,考察了他巴唑模型的体温、体重变化、血清皮质醇(CORT)、三碘甲状腺原氨酸(T3)、甲状腺素(T4)、睾酮(T)/雌二醇(E2)等指标,观察模型下丘脑-垂体-肾上腺轴、甲状腺轴、性腺轴个轴的变化,并比较雄性大鼠和雌性大鼠模型的区别。结果:认为大鼠他巴唑模型可以出现明显的体温下降,血清T3、T4下降,而对肾上腺轴和性腺轴的干扰不明显。结论:他巴唑模型适用于模拟甲状腺功能低下为主的肾阳虚证,其他方面的病理改变不明显,雄性大鼠和雌性大鼠均可以用作造模动物。

Review the Effect of Hypothalamus-pituitary Adrenal Hypothyroid and Gonad on Thiamazole Models

Objective:Review the effect of thiamazole model on hypothal amus-pituitary adrenal,hypothyroid and gonad.Methods:Use the most common used period(15days)of thiamazole model build models.There are three groups of different dosages.Research the temperature,avoirdupois,cortisol(COR),triiodothyronine(T_3),thyroxine(T_4),estroron(E_2),testosterone(T),etc.Review the effect of thiamazole model on hypothal amus-pituitary adrenal,hypothyroid,gonad,and compare the difference between two genders.Results:There are obvious descend in triiodothyronine(T_3)and thyroxine(T_4).No evidence effect on adrenal and gonad is found in the models.Conclusion:Thiamazole models are suitable for mimic kidney-yang deficiency that is mostly arosed by insufficiency of thyriod hormones.There are no obvious change the other two axis.Male or female rat are both suit for make thiamazole model.