| 二乙酰一肟-氨基硫脲比色法测定酶转化液中的L-瓜氨酸 |
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| 引用本文： | 钱嘉南,孙志浩,刘宇鹏,孟繁君,刘咏梅. 二乙酰一肟-氨基硫脲比色法测定酶转化液中的L-瓜氨酸[J]. 中国医药工业杂志, 2007, 38(7): 519-522 |
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| 作者姓名： | 钱嘉南 孙志浩 刘宇鹏 孟繁君 刘咏梅 |
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| 作者单位： | 江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡,214036 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;教育部长江学者和创新团队发展计划 |
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| 摘 要： | 用二乙酰一肟-氨基硫脲比色法测定酶转化液中的L-瓜氨酸含量,并研究了测定波长、氨基硫脲用量、铁离子等因素的影响。线性范围为0.1～20mg/L（r=0.999）,回收率99.8%。
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| 关 键 词： | 二乙酰一肟 氨基硫脲 L-瓜氨酸 比色法 测定 |
| 文章编号： | 1001-8255（2007）07-0519-04 |
| 修稿时间： | 2006-11-27 |
Determination of L-Citrulline in Enzymatic Conversion Solution by Diacetylmonoxime-Thiosemicarbazide Colorimetry |
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| QIAN Jia-nan,SUN Zhi-hao,LIU Yu-peng,MENG Fan-jun,LIU Yong-mei. Determination of L-Citrulline in Enzymatic Conversion Solution by Diacetylmonoxime-Thiosemicarbazide Colorimetry[J]. , 2007, 38(7): 519-522 |
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| Authors: | QIAN Jia-nan SUN Zhi-hao LIU Yu-peng MENG Fan-jun LIU Yong-mei |
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| Affiliation: | School of Biotechnology, Southern Yangtze University, Key Lab. of Industry Biotechnology, Ministry of Education, Wuxi 214036 |
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| Abstract: | A diacetylmonoxime-thiosemicarbazide colorimetry was established for determination of L-citrulline in enzymatic conversion solution. The in? uence factors including absorption wavelength, thiosemicarbazide concentration and Fe3 were investigated. The linearity range was 0.1 -20mg/L (r = 0.999), and the average recovery was 99.8%. |
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| Keywords: | diacetylmonoxime thiosemicarbazide L-citrulline colorimetry determination |
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