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Identification of Histoplasma capsulatum from culture extracts by real-time PCR
Authors:Martagon-Villamil Jose  Shrestha Nabin  Sholtis Mary  Isada Carlos M  Hall Gerri S  Bryne Terry  Lodge Barbara A  Reller L Barth  Procop Gary W
Affiliation:Division of Infectious Diseases. Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Abstract:We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
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