白细胞滤除对新鲜冰冻血浆质量影响的研究

目的探讨白细胞滤除对新鲜冰冻血浆（FFP）质量的影响。方法32例新鲜冰冻血浆解冻后滤除白细胞,检测滤除前后血浆容积,血浆中蛋白、Ⅴ因子、Ⅷ因子、纤维蛋白原（Fg）以及补体的含量变化。结果血浆中白细胞滤除率均〉99.9%;滤除白细胞前后血浆容积回收率无明显变化（P〉0.05）;血浆蛋白含量,血浆中Ⅴ因子、Ⅷ因子、Fg和补体含量无明显差异（P〉0.05）;去白细胞血浆的血培养检测均未见细菌生长。结论新鲜冰冻血浆解冻后迅速进行白细胞滤除能有效去除绝大多数白细胞及其复合物,对血浆中的蛋白及凝血因子含量无显著影响。

Effect of leukocytes removal on the quality of the fresh frozen plasma

Objective To evaluate whether the removal of leukocytes from the fresh frozen plasma affects its quality. Methods Totally, 32 bags of fresh frozen plasma were examined. Briefly, after thawing, the leukocytes were removed from the plasma, and the change of plasma volume, the level of plasma protein, Ⅴ factor, Ⅵ factor, fibrinogen and complement were measured before and after leukocytes removal. Results Consistent with the international reference standard, the efficiency of leukocyte removal was above 99.9 %. There was no significant change in the plasma volume before and after leukocytes filtration (P ＞ 0.05). There were also no significant changes in the level of plasma proteins, Ⅴ factor, Ⅷ factor, fibrinogen and complement before and after leukocytes filtration (P ＞ 0.05). No bacteria were detected by hemoculture of all the plasma. Conclusions Leukocytes filtration immediately after thawing of the fresh frozen plasma, can efficiently remove the majority of leukocytes and their composites. There are no significant changes in the level of plasma proteins and blood coagulation factors during this process.