PMN apoptosis and its relationship with the lung injury after chest impact trauma |
| |
Authors: | Liu Ren Li Shuang-ding Min Jia-xin Xiao Nan Jiang Qi-sheng Tian Kun-lun Diao You-fang |
| |
Institution: | 1. Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China 2. Medical College of Xi'an Jiaotong University, Xi'an 710061, China 3. Xinqiao Hospital, Third Military Medical University, Chongqing 400038, China |
| |
Abstract: | Background Polymorphonuclear neutrophil (PMN),one of the most important inflammatory cells,functions throughout the initiation,progression and resolution of inflammation. This study aimed at investigating the relationship between PMN apoptosis and the lung injury after chest impact trauma. Methods PMNs were purified from rabbits subjected to the chest impact trauma and their apoptosis,necrosis,survival and respiratory burst were detected by flow cytometry. Meanwhile,lactate dehydrogenase and (LDH) Ca 2 ]i were measured. Results The delayed apoptosis of PMNs in bronchoalveolar lavage fluid was observed from 2 hours to 12 hours after trauma,and viable cells increased. Respiratory burst of PMNs in bronchoalveolar lavage fluid was increased significantly from 2 hours with the peak at 8 hours. Meanwhile,lactate dehydrogenase in bronchoalveolar lavage fluid was higher than that in control ( P <0.05) from 4 hours to 24 hours,and intracellular free Ca 2 in PMN was increased temporarilly. Conclusions Retention of PMN in tissues and the abnormality in apoptotic pathway inevitably generate persistent activation of PMN and excessive release of toxic substances,resulting in tissue injury. The temporary increase of intracellular free Ca 2 may be responsible for the delayed apoptosis of PMN. |
| |
Keywords: | chest impact trauma polymorphonuclear neutrophil apoptosis respiratory burst secondary lung injury |
本文献已被 CNKI 万方数据 PubMed 等数据库收录! |
| 点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息 |
| 点击此处可从《中华医学杂志(英文版)》下载免费的PDF全文 |
|