改良三袖套吻合法建立大鼠左肺原位移植模型及其鉴定

目的对三袖套吻合法建立的大鼠肺移植模型进行改进,建立操作简便和稳定有效的大鼠左肺原位移植模型。方法在预实验的基础上,对三袖套吻合法建立的大鼠肺移植模型进行以下改进:供体采用胸骨正中切口,经肺动脉圆锥处进行低压肺灌注;制作袖套时用无损伤血管夹固定套管尾部;套入袖套时用无创显微心耳钳钳夹住左肺门,在不离断病肺的情况下完成吻合;完善呼吸道管理流程。以手术成功率、手术时间、移植肺功能、肺组织病理学改变等对模型进行评估。结果完成大鼠左肺原位移植10例,手术成功率80%。供肺灌洗到获取时间为(12±2)min,体外套管时间为(18±3)min,吻合时间为(31±3)min。移植肺再灌注15 min的氧合指数显著高于单肺通气30 min(P<0.05)。移植肺再灌注2 h后的病理学改变符合典型的缺血-再灌注损伤特征。结论成功改进了大鼠左肺原位移植模型,该模型稳定有效且操作简便。

Improvement of rat model of orthotopic left lung transplantation

Objective To improve the cuff-like vessel anastomosis technique-based rat model of lung transplantation,and establish a simple and stable rat model of orthotopic left lung transplantation. Methods Based on preliminary experiments,improvement was made from cuff-like vessel anastomosis technique-based rat model of lung transplantation on the following aspects: lungs were extracted from donors through median sternotomy,and were perfused through cut at the cone of the pulmonary artery with low pressure;blood ve...