| 聚古罗糖醛酸硫酸酯-铜纳米胶束制备及抗肿瘤活性研究 |
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| 引用本文： | 王定福,仇晓雷,郝丽桦,范子瑞,王世欣,李春霞. 聚古罗糖醛酸硫酸酯-铜纳米胶束制备及抗肿瘤活性研究[J]. 中国海洋药物, 2022, 41(2): 55-61 |
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| 作者姓名： | 王定福 仇晓雷 郝丽桦 范子瑞 王世欣 李春霞 |
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| 作者单位： | 中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室;青岛海洋生物医药研究院,中国海洋大学 海洋药物教育部重点实验室 |
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| 基金项目： | 山东省重大科技创新专项(2018SDKJ0404)资助 |
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| 摘 要： | 目的 制备1种基于化学动力学疗法的聚古罗糖醛酸硫酸酯(PGS)-铜纳米胶束，进行抗肿瘤活性研究。方法 采用聚离子复合法，将PGS与铜离子及过氧化氢反应制备聚电解质纳米胶束PGS-Cu，分光光度法测定铜离子含量和羟基自由基，CCK-8法测定细胞存活率。结果 制备的PGS-Cu纳米粒径为(109.54±14.29) nm，分布系数为0.23±0.02,Zeta电位为(-47.58±1.71) mV。酸性环境下，PGS-Cu纳米胶束铜离子累积释放量显著高于中性环境，并产生·OH。100μg·mL-1的PGS-Cu纳米胶束作用下，人源三阴性乳腺癌细胞(MDA-MB-231)、小鼠乳腺癌细胞(4T1)和小鼠正常成纤维细胞(NIH/3T3)的存活率分别为14.87%、62.24%和90.77%，表明PGS-Cu纳米胶束对乳腺癌细胞有显著的细胞毒性，且对MDA-MB-231细胞抑制效果最佳。此外，4T1细胞在pH 7.4和6.0时的存活率分别为62.24%和18.33%，说明该纳米胶束具有pH响应性。结论 成功构建了1种基于化学动力学疗法、以多糖为载体的聚电解纳米胶束，该纳米胶...
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| 关 键 词： | 聚古罗糖醛酸硫酸酯 聚电解质纳米胶束 化学动力学疗法 抗肿瘤 |
| 收稿时间： | 2021-05-06 |
| 修稿时间： | 2021-05-17 |
Preparation and antitumor activity of polyguluronate sulfate-copper nanomicelles |
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| WANG Ding-fu,QIU Xiao-lei,HAO Li-hu,FAN Zi-rui,WANG Shi-xin and. Preparation and antitumor activity of polyguluronate sulfate-copper nanomicelles[J]. Chinese Journal of Marine Drugs, 2022, 41(2): 55-61 |
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| Authors: | WANG Ding-fu QIU Xiao-lei HAO Li-hu FAN Zi-rui WANG Shi-xin and |
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| Affiliation: | Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China;Marine Biomedical Research Institute of Qingdao,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China |
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| Abstract: | |
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| Keywords: | Polyguluronate sulfate Polyelectrolyte nanomicelles Chemodynamic therapy Antitumor |
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