| IL-18真核表达载体的构建及其在HO-8910细胞株的表达 |
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| 引用本文： | 吴晓莉,张林杰,许健. IL-18真核表达载体的构建及其在HO-8910细胞株的表达[J]. 蚌埠医学院学报, 2008, 33(2): 144-146 |
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| 作者姓名： | 吴晓莉 张林杰 许健 |
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| 作者单位： | 1. 安徽医科大学免疫学教研室, 安徽合肥 230032;2. 浙江中医药大学生命科学学院, 浙江杭州 310053 |
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| 基金项目： | 浙江省科技攻关计划项目重点科研社会发展项目 |
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| 摘 要： | 目的:通过构建人白细胞介素18(IL-18)真核表达载体,观察其在HO-8910细胞株的表达情况。方法:应用分子克隆技术将IL-18基因插入pCI真核表达载体,应用脂质体介导法转染人卵巢癌HO-8910细胞株。转染分3组,空白组:人卵巢癌HO-8910细胞株+脂质体;对照组:卵巢癌HO-8910细胞株+pCI;实验组:卵巢癌HO-8910细胞株+pCI-hIL-18。以RT-PCR方法分别检测3组的IL-18表达情况。结果:成功构建pCI-hIL-18真核表达载体并转染HO-8910细胞株,RT-PCR、ELISA显示转染pCI-hIL-18重组质粒组在转染的细胞内获得表达。结论:IL-18基因能够转染到HO-8910细胞株中并获得表达。
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| 关 键 词： | 卵巢肿瘤 白细胞介素-18 基因 免疫应答 HO-8910细胞株 质粒 |
| 文章编号： | 1000-2200(2008)02-0144-03 |
| 收稿时间： | 2007-10-12 |
| 修稿时间： | 2007-10-12 |
Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line |
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| WU Xiao-li,ZHANG Lin-jie,XU Jian. Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line[J]. Journal of Bengbu Medical College, 2008, 33(2): 144-146 |
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| Authors: | WU Xiao-li ZHANG Lin-jie XU Jian |
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| Affiliation: | 1. Department of Immunology, Anhui Medical University, Hefei Anhui 230032;2. Life Scince College, Zhejiang Chinese Medical University, Hangzhou Zhengjiang 310053, China |
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| Abstract: | Objective:To constrct the eukaryotic expression vector of interleukin-18(IL-18)and study its expression in HO-8910 cell line.Methods:Interleukin-18 gene was inserted into the eukaryotic expression vector pCI with molecular cloning technique,transferred to HO-8910 cell line with Lipotectamine.Three transferred groups were studied:HO-8910+Lipotectamine,HO-8910+pCI and HO-8910 + pCI-hIL-18.The expression of IL-18 gene was comfirmed by RT-PCR technique.Results:The eukaryotic expression vector of IL-18(pCI-hIL-1... |
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| Keywords: | ovarian neoplasms interleukin-18 genes immune response HO-8910 cell line plasmids |
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