| 固相萃取-高效液相色谱法测定人血浆中雷尼替丁浓度 |
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| 引用本文： | 薛晓燕,朱荣华,彭文兴,李焕德.固相萃取-高效液相色谱法测定人血浆中雷尼替丁浓度[J].国际医药卫生导报,2011,17(4):455-459. |
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| 作者姓名： | 薛晓燕 朱荣华 彭文兴 李焕德 |
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| 作者单位： | 薛晓燕 (东莞市石龙人民医院药剂科,511700) ； 朱荣华 (中南大学湘雅二医院临床药学研究室,410011) ； 彭文兴 (中南大学湘雅二医院临床药学研究室,410011) ； 李焕德 (中南大学湘雅二医院临床药学研究室,410011) ； |
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| 摘 要： | 目的 建立测定人血浆中雷尼替丁浓度的HPLC-UV方法.方法 血浆经C18柱固相萃取,洗脱液在高效液相色谱仪上测定.结果 血浆雷尼替丁浓度在10.0～1000.0ng·ml-1之间线性关系良好,相关系数r2为0.999.日内变异和日间变异在3.6%之内,萃取回收率在87.2%～96.2%之间.结论 本方法操作简便、快速、准确,适合于雷尼替丁的人体药代动力学研究.
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| 关 键 词： | 雷尼替丁 HPLC-UV 血浆 药动学 |
Dertermination of Ranitidine in Human Plasma by Solid phase Extraction and HPLC |
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| XUE Xiao-yan,ZHU Rong-hua,PENG Wen-xing,LI Huan-de.Dertermination of Ranitidine in Human Plasma by Solid phase Extraction and HPLC[J].International Medicine & Health Guidance News,2011,17(4):455-459. |
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| Authors: | XUE Xiao-yan ZHU Rong-hua PENG Wen-xing LI Huan-de |
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| Institution: | (Shilong people＇s hospital of Dongguan, Dongguan 511700, China) |
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| Abstract: | Objective To make a sensitive method for the determination of ranitidine in human plasma by HPLC-UC. Methods Plasma samples were extracted with C18 Solid phase columns. Chromatograph analysis was carried out with a C18 column and UV Detector which was set at 316 nm. Results Interior impurity in human plasma does not interfere with the determination of ranitidine and internal standard. Good linear relationship(r2=0.999) was obtained in the concentration range from 10.0 to 1000.02%. Conclusion This method is sensitive, selective and simple, is suitable for clinical pharmacokinetic and bioequivalent study of ranitidine. |
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| Keywords: | HPLC-UV |
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