磷酸二酯酶7B在慢性淋巴细胞白血病患者中的表达及预后意义

目的 为了研究磷酸二酯酶(PDE)7B在慢性淋巴细胞白血病(CLL)患者中的表达及临床意义.方法 应用实时定量逆转录PCR(qRT-PCR)技术检测87例未治疗CLL患者和20例正常人中PDE7B的表达水平,并分析其与CLL临床预后指标的关系.结果 87例CLL患者PDE7B表达水平中位数为5.4×10-4(0.2×1...

Expression of Cyclic Nucleotide Phosphodiesterase 7B in Patients with chronic lymphocytic leukemia and Its Prognostic Significance

Objective To investigate the expression level of cyclic nucleotide phosphodiesterase (PDE) 7B mRNA in chronic lymphocytic leukemia (CLL) and its prognostic value of PDE7B in CLL.Methods A total of 87 blood samples from untreated CLL patients were collected, real-time quantitative RT-PCR (qRT-PCR) was used to access PDE7B mRNA expression, and its association with prognostic index was analyzed by statistics software. Results The median values of PDE7B mRNA expression level in 87 CLL patients and normal control were 5.4 × 10-4 (0. 2 × 10-4 ～ 1.5 × 10-2) and 0.5 × 10-4 (0.18 × 10-4～ 1. 7 × 10-4), respectively (P ＜ 0. 001). The results showed that PDE7B mRNA expression was significantly associated with age, Binet stages, immunoglobulin heavy-chain variable region (IgVH)mutation status, CD38 expression and cytogenetic abnormalities, but not with gender and ZAP-70expression. PDE7B mRNA expression in 26 CLL patients who had treatment indications and received treatment was higher than untreated ones (P = 0. 021). Conclusion PDE7B mRNA expression was markedly higher in CLL patients compared with normal controls, which was significantly correlates with unfavorable cytogenetic characteristics, Binet stages B and C, unmutated IgVH and CD38 positive. It might be a novel prognostic indicator in CLL, which possess important clinical significance.