测定脐血血清游离氨基酸评估新生儿营养状况

目的 :测定并建立本地区新生儿脐血氨基酸的正常值 ,探讨其变化规律及作为营养指标的科学性与可行性。方法 :2 0 0 3年 9～ 11月随机抽取大连市妇产医院自然分娩新生儿 82名 (适于胎龄儿 5 0名、小于胎龄儿 9名、大于胎龄儿 2 3名 ) ,孕龄 39.4 5± 1.2 5周 ,出生体重 345 6 .0 9± 4 2 7.19g。采用高效液相色谱 ,AccQ .Tag柱法测量脐血血清游离氨基酸含量。结果 :AccQ .Tag法使标准品和样品的所有峰得到良好的分离 ,脐血氨基酸总含量分别为适于胎龄儿组 4 .0 88± 2 .35 6 9μmol/ml,小于胎龄儿组 2 .6 711± 1.6 96 6 μmol/ml,大于胎龄儿组 4 .0 374± 1.882 5 μmol/ml。结论 :高效液相色谱方法测定氨基酸准确 ,可靠 ,重现性好 ,此方法分析脐血氨基酸是可行的。脐血氨基酸有望成为评估胎儿宫内营养状况的生化指标。对不同胎龄儿组氨基酸的相关性比较推测 ,胎儿的宫内营养状况可能与母血营养的协调供给有关

Assessment On Nutrition Status Of Newborn Infants by Measurement Of Free Amino Acid Of Umbilical Serum

Objective: To establish the accurate method for measure the amino acid of the umbilical blood to determine and set up the range of normal value for amino acid of the umbilical blood for the full-pregnancy infants, and to explore the changing rules, scientific property and feasibility as the nutrition indicators. Methods:82 cases of natural birth infants, whose gestational age were (39.45±1.25) weeks and the born weight were (3456.09±427.19) g, were selected randomly from September to November in 2003. The amount of free amino acid of umbilical serum was measured by HPLC AccQ.Tag package method. Results:The amount of amino acid of umbilical blood was 4.088±2.3569 umol/ml in appropriate gestational age fetuses, 2 6711±1 6966 umol/ml in small gestational age fetuses and 4 0374±1 8825 umol/ml in lager gestational age fetuses. Conclusion:The HPLC is accurate and reliable way of measuring amount of amino acid. The FAA in umbilical blood is possible to be an indicators for evaluating nutrition conditions of fetus.It is estimated that nutrition conditions of the fetus might be related to the supply of nutrition of maternal blood.