| Survivin反义寡核苷酸联合足叶乙甙诱导K562细胞株凋亡的研究 |
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| 引用本文： | 李建厂,徐酉华,于洁,温贤浩. Survivin反义寡核苷酸联合足叶乙甙诱导K562细胞株凋亡的研究[J]. 中国优生与遗传杂志, 2008, 16(10) |
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| 作者姓名： | 李建厂 徐酉华 于洁 温贤浩 |
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| 作者单位： | 滨州医学院附属医院儿科 重庆医科大学附属儿童医院血液科 |
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| 摘 要： | 目的探讨Survivin反义寡核苷酸及VP-16对K562细胞株凋亡的影响。方法将Survivin反义寡核苷酸通过脂质体转染K562细胞株,用免疫组化法测定转染前后K562细胞survivin基因及Caspase-3的表达差异,并加入足叶乙甙,用Tenu l及Annexin V-FITC测定各组细胞的凋亡率。结果转染后survivin基因表达下降,Caspase-3的表达升高,K562细胞生长受抑,细胞凋亡率升高;联合反义寡核苷酸与足叶乙甙,细胞凋亡率明显增加。结论Survivin反义寡核苷酸能够促进白血病细胞株K562的凋亡,能够提高K562对足叶乙甙的敏感性。
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| 关 键 词： | Survivin 反义寡核苷酸 K562细胞 足叶乙甙 凋亡 |
Study on the apoptosis of K562 cells treated with antisense oligonucleotide targeting survivin and Etoposide |
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| Affiliation: | LI Jian-chang1; XU You-hua2; et al.(1.Dept.of Pediatrics; Affiliated Hospital; Binzhou Medical College; Shandong 256603 2.Division of Hematology/Oncology Children′s Hospital Chongqing University of Medical Science; Chongqing China; 400014); |
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| Abstract: | Objective: To study the apoptosis of leukemia cells induced by antisense oligonucleotide targeting survivin and etoposide(VP-16).Methods: Oligonucleotides were delivered in the form of complexes with Lipofectin.Then treated with VP16.Examined The expression of survivin Caspse-3 by immunohistochemistry(SABC).And detected the apoptotic rate of each group with two methods: TUNEL assay and Annexin V-FITC assay.Results: The protein of survivin is overexpress in K562 cells,but after treated with antisense oligonu... |
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| Keywords: | Survivin |
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