高效毛细管电泳法测定宁夏枸杞中N-反式阿魏酸酪酰胺的含量

 目的 采用高效毛细管电泳法,建立宁夏枸杞中N-反式阿魏酸酪酰胺含量测定方法。方法 未涂层毛细管柱（50 cm×75 μm,有效长度为40 cm）,以50 mmol·L^-1硼砂溶液（pH 9.0,磷酸调节）为运行缓冲液,运行电压为20 kV,柱温25 ℃,检测波长214 nm。结果 在进样量0.25～15 μg·mL^-1内线性关系良好,相关系数r=0.999 8（n=7）。N-反式阿魏酸酪酰胺的平均回收率为101.8%,RSD为2.06%。结论 该方法准确可靠,可用于测定宁夏枸杞中N-反式阿魏酸酪酰胺的含量,为宁夏枸杞开发利用提供理论依据。

Determination of N-Trans-Feruloyl Tyramine in Lycii Fructus by HPCE

OBJECTIVE To establish an HPCE method for determination of N-trans-feruloyl tyramine in Lycii Fructus. METHODS The chromatographic separation was performed on an uncoated fused silica capillary of 50 cm×75 μm ID (40 cm of effective length). 50 mmol·L^-1 Na₂B₄O₇ (pH 9.0 with H₃PO₄) was selected as the running buffer. The applied voltage was 20 kV, and the column temperature was maintained at 25 ℃. The chromatogram was detected at 214 nm. RESULTS The linearity for N-trans-feruloyl tyramine was in a range of 0.25-15 μg·mL^-1 (r=0.999 8, n=7). The average recovery for N-trans-feruloyl tyramine was 101.8% with RSD of 2.06%. CONCLUSION The method is accurate, reliable, and can be used as a quality control method for N-trans-feruloyl tyramine in Lycii Fructus, which can provide basis for development and utilization of Lycii Fructus.