一测多评法测定银黄制剂中4种黄酮类成分含量

 目的 建立一测多评法测定银黄制剂中黄芩黄酮类成分含量的分析方法，证明一测多评法用于复方制剂的可行性。方法 以银黄制剂为研究对象，建立汉黄芩苷（wogonoside）、黄芩素（baicalein）和汉黄芩素（wogonin）与黄芩苷（baicalin）间的相对校正因子。分别采用外标法和一测多评法测定银黄制剂中4种黄酮类成分的含量，并将一测多评法的计算值与外标法实测值用相对误差进行比较。结果 黄酮类成分间的相对校正因子分别为f ^{274 nm}_{汉黄芩素/黄芩苷}=1.20，f ^{274 nm}_{黄芩素/黄芩苷}=1.62，f ^{274 nm}_{汉黄芩素/黄芩苷}=1.68。一测多评法的计算结果与外标法的实测值之间没有显著性差异，实验所得的相对校正因子可信。结论 一测多评法可作为一个新的质量评价模式用于银黄制剂中黄酮类成分的含量测定。

Simultaneous Determination of Four Flavones in Yinhuang Preparations by QAMS

OBJECTIVE To establish a quality evaluation method,quantitative analysis of multi-components with a single-marker(QAMS),to simultaneously determine the contents of four flavones in Yinhuang preparations. METHODS Baicalin was used as the internal reference substance.The relative correlation factors(RCF) of wogonoside,baicalein and wogonin to baicalin were calculated and established.The contents of these four flavones were determined by the external standard method and QAMS respectively.The QAMS method was validated through comparison of the results obtained by the two different methods. RESULTS Within the linear ranges,the values of RCF determined at 274 nm of wogonoside,baicalein and wogonin to baicalin were 1.20,1.62 and 1.68 respectively.The RCF showed good reproducibility within the range of 1.7%-4.8% for the three studied compounds.The two methods did not show significant difference in assay results. CONCLUSION The QAMS method is feasible,credible,and can be used to determine multiple flavones in Yinhuang preparations.