| Effect of HCMV IE1 Protein on Cytokines Secretion and Apoptosis of Macrophages |
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| 引用本文： | 陈琳 万艳平 陈熙 刘安元 朱翠明 余敏君 曹清香. Effect of HCMV IE1 Protein on Cytokines Secretion and Apoptosis of Macrophages[J]. 中国癌症研究, 2008, 20(1): 12-16. DOI: 10.1007/s11670-008-0012-5 |
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| 作者姓名： | 陈琳 万艳平 陈熙 刘安元 朱翠明 余敏君 曹清香 |
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| 作者单位： | Institute of Pathogenic Biology, University of South China, Hengyang 421001 |
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| 基金项目： | This work was supported by the Education Committee of Hunan Province （No. 05C472）. |
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| 摘 要： | Objective： To investigate the effect of human cytomegalovirus （HCMV） IE1 protein on the secretory activity and apoptosis of macrophages. Methods： The eukaryotic expression vector pEGFP-C1/IE1 was used to transfect THP-1-macrophages. 48 h after transfection, the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope. The levels of IL-1β and TNF-α in the culture media were examined by ELISA, and the mRNA expression of them was analyzed by RT-PCR. Cell undergoing apoptosis were determined by flow cytometry using the propidium iodide （PI） staining method. The data were analyzed by SPSSI3.0. Results： As observed under fluorescent microscope, the expressions of GFP-IEI and GFP by plasmid pEGFP-C1/IE1 or pEGFP-C1 in THP-1-macrophages could be found in nuclei or whole cells. Conclusion： As demonstrated by RT-PCR and ELISA, mRNA and protein expressions of IL-1β and TNF-α and promotes apoptosis in THP-1-macrophages.
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| 关 键 词： | 人类巨细胞 巨噬细胞 IL-1β TNF-α 细胞凋亡 |
| 文章编号： | 1000-9604（2008）01-0012-05 |
| 收稿时间： | 2007-06-06 |
| 修稿时间： | 2007-11-18 |
Effect of HCMV IE1 protein on cytokines secretion and apoptosis of macrophages |
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| Lin Chen,Yan-ping Wan,Xi Chan,An-yuan Liu,Cui-ming Zhu,Min-jun Yu,Qing-xiang Cao. Effect of HCMV IE1 protein on cytokines secretion and apoptosis of macrophages[J]. Chinese Journal of Cancer Research, 2008, 20(1): 12-16. DOI: 10.1007/s11670-008-0012-5 |
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| Authors: | Lin Chen Yan-ping Wan Xi Chan An-yuan Liu Cui-ming Zhu Min-jun Yu Qing-xiang Cao |
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| Affiliation: | (1) Institute of Pathogenic Biology, University of South China, Hengyang, 421001, China |
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| Abstract: | Objective:To investigate the effect of human cytomegalovirus(HCMV)IE1 protein on the secretory activity and apoptosis of macrophages. Methods:The eukaryotic expression vector pEGFP-C1/IE1 was used to transfect THP-1-macrophages.48 h after transfection,the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope.The levels of IL-1β and TNF-α in the culture media were examined by ELISA,and the mRNA expression of them was analyzed by RT-PCR.Cell undergoing apoptosis were determined by flow cytometry using the propidium iodide(PI) staining method.The data were analyzed by SPSS13.0. Results:As observed under fluorescent microscope,the expressions of GFP-IE1 and GFP by plasmid pEGFP-C1/IE1 or pEGFP-C1 in THP-1-macrophages could be found in nuclei or whole cells. Conclusion:As demonstrated by RT-PCR and ELISA,mRNA and protein expressions of IL-1β and TNF-α and promotes apoptosis in THP-1-macrophages. |
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| Keywords: | Human cytomegalovirus(HCMV) IE1 Macrophages Apoptosis |
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