罗格列酮对慢性环孢素肾病大鼠肾间质纤维化的保护作用

目的 探讨罗格列酮(RGZ)对环孢素A(CsA)所致慢性环孢素肾病(CCN)大鼠肾间质纤维化的保护作用.方法 28只健康雄性SD大鼠被随机分成4组:(1)对照组(n=6):低盐饮食(LSD); (2)RGZ组(n=6):LSD+RGZ(5 mg·kg-1·d-1)；(3)CsA组(n=8):LSD+CsA(15 mg·kg-1·d-1),(4)RGZ+CsA组(n=8):LSD +CsA(15 mg·kg-1·d-1)+RGZ(5 mg·kg-1 ·d-1).实时荧光定量PCR观察骨调素(OPN)及RANTES表达量；RT-PCR法检测MMP-9和TIMP-1的表达量；常规染色观察肾脏病理改变.结果 RGZ可改善CsA所致大鼠肌酐清除率的降低(0.253±0.027)比(0.133±0.018) ml/min,P＜0.05]；降低CCN大鼠肾间质单个核细胞浸润(22.50±2.71)比(30.38±3.11),P＜0.05]；减轻肾间质纤维化程度(1.707±0.019)比(2.335±0.022),P＜0.05].与CsA组比较,RGZ+CsA组大鼠肾组织中OPN和RANTES mRNA表达量和MMP-9、TIMP-1 mRNA表达量降低(均P＜0.05).结论 RGZ可能通过下调趋化因子OPN、RANTES及MMP-9、TIMP-1的表达,改善CCN大鼠肾间质纤维化.

Rosiglitazone reduces renal interstitial fibrosis in chronic cyclosporine nephropathy rats

Objective To investigate the effect of rosiglitazone on renal interstitial fibrosis in chronic cyclosporine nephropathy (CCN) rats. Methods Twenty-eight rats were randomly assigned to control group, rosiglitazone (RGZ, 5 mg&#8226;kg-1&#8226;d-1) group, cyclosporine A(CsA, 15 mg&#8226;kg-1&#8226;d-1) group, rosiglitazone(5 mg&#8226;kg-1&#8226;d-1) +CsA group. Real-time PCR and RT-PCR methods were used to investigate the expressions of OPN, RANTES on the 14th day and MMP-9, TIMP-1 on the 35th day in kidney of CCN respectively. Results In comparison with control group, the expressions of OPN, RANTES, MMP-9, TIMP-1 in CsA and RGZ+CsA groups were increased (P<0.05). In comparison with the CsA group , the expressions of OPN, RANTES, MMP-9, TIMP-1 in CsA+RGZ group significantly decreased (P<0.05). Conclusion Rosiglitazone may protect renal tissue after CCN by decreasing expressions of OPN, RANTES, MMP-9, TIPM-1.