镨三元配合物对HL60细胞生长代谢热动力学的影响

目的观察水杨酸硫代脯氨酸镨三元配合物{［Pr（C7H5O3）2（C4H6NO2S）］•2H2O }对体外培养HL 60细胞生长代谢热动力学的影响。方法应用微量量热法绘制［Pr（C7H5O3）2（C4H6NO2S）］•2H2O作用时HL 60细胞生长代谢产热曲线，解析生长代谢速率常数（κ）、最大热输出功率（Pmax）、达峰时间（tmax）及配合物对细胞生长代谢的抑制率（I） 等热动力学参数，实验72 h应用显微观测法检查细胞凋亡情况。结果不同浓度［Pr（C7H5O3）2（C4H6NO2S）］•2H2O处理HL 60细胞后，细胞生长代谢受到不同程度抑制，并呈浓度依赖性；［Pr（C7H5O3）2（C4H6NO2S）］•2H2O达到4.0 μg•mL－1时，肿瘤细胞κ和Pmax明显减小，I明显增大。结论［Pr（C7H5O3）2（C4H6NO2S）］•2H2O影响HL 60细胞生长代谢，且具有剂量 效应关系，其有效作用浓度为4.0 μg•mL－1。

Thermodynamic Effect of the Ternary Complexes of Praseodymium with Salicylic Acid and 8-hydroxy Quinoline on Proliferation of HL-60 Cells

Objective To investigate the thermodynamic effect of the ternary complexes of praseodymium with salicylic acid and 8-hydroxy quinoline[Pr(C7H5O3)2(C4H6NO2S)·2H2O] on the proliferation of HL-60 cells in vitro.Methods Microcalorimetry was performed to determine the curve of cell proliferation and metabolic heat after the coordination compound was added.Thermodynamic parameters [growth and metabolism rate constant(κ),maximum heat output power(Pmax),peak time(tmax) and cell growth inhibition rate(I)] were analyzed.Results The proliferation activity of HL-60 cells was inhibited in a dose-dependent manner when treated with different concentrations of [Pr(C7H5O3)2(C4H6NO2S)]·2H2O.When the cells were treated with 4.0 μg·mL-1 [Pr(C7H5O3)2(C4H6NO2S)]·2H2O,the κ and Pmax decreased significantly,and the I significantly increased.When the cells were treated with >4.0 μg·mL-1 [Pr(C7H5O3)2(C4H6NO2S)]·2H2O,apoptotic cells were seen.Conclusion [Pr(C7H5O3)2(C4H6NO2S)]·2H2O can affect the growth and metabolism of HL-60 cells in a dose-dependent manner,and its effective concentration is 4.0 μg·mL-1.