强的松对塞克硝唑在大鼠体内的药物动力学行为的影响

目的建立测定大鼠血浆中塞克硝唑的HPLC法,并用于考察联合使用强的松后塞克硝唑在大鼠体内药动学行为的改变。方法 12只健康雄性SD大鼠随机分成2组(分别为单独和联合给药组),采用HPLC法测定血浆中塞克硝唑的浓度。流动相为乙腈-0.02 mol.L-1磷酸二氢钾(体积比为25∶75,pH 3.0),流速为1 mL.min-1,检测波长为320 nm。结果大鼠单独灌胃给予塞克硝唑与联合强的松给药组主要的的药动学参数:ρmax分别为(95.2±3.7)和(97.6±14.9)mg.L-1,tmax分别为(0.67±0.26)和(0.79±0.46)h,t1/2分别为(3.71±0.73)和(4.47±1.68)h,AUC0-t分别为(587±50)和(663±72)mg.h.L-1,AUC0-∞分别为(592±49)和(669±73)mg.h.L-1,均无显著性差异(P>0.05)。结论强的松对塞克硝唑在大鼠体内的药动学行为无显著影响。

Effect of prednisone on pharmacokinetics of secnidazole in rats

Objective To establish an HPLC method for determination of secnidazole in rat plasma and investigate the effect of prednisone on pharmacokinetics of secnidazole.Methods Twelve healthy male SD rats were divided into two groups(i.g.administration of secnidazole alone and combination of secnidazole and prednisone).The concentration of secnidazole in plasma was determined by HPLC.The mixture of acetonitrile-0.02 mol · L-1 potassium dihydrogen phosphate buffer(V∶ V=25∶ 75,pH 3.0)was used as mobile phase with a flow rate of 1 mL · min-1.The detecting wavelength was maintained at 320 nm.Results The main pharmacokinetic parameters of single and combination administration groups were as follow:ρmax were(95.2±3.7)and(97.6±14.9)mg · L-1,tmax were(0.67±0.26) and(0.79±0.46)h,t1/2 were(3.71±0.73)and(4.47±1.68)h,AUC0-t were(587±50)and(663±72)mg · h · L-1;AUC0-∞ were(592±49)and(669±73)mg · h · L-1,respectively,which showed no significant difference between the two groups(P> 0.05).Conclusions There is no effect on pharmacokinetics of secnidazole after combination administration with prednisone in rats.