胶束电动毛细管色谱法测定金果榄中古伦宾和蝙蝠葛任碱的含量

目的建立测定金果榄药材中古伦宾和蝙蝠葛任碱含量的胶束电动毛细管色谱法。方法采用未涂层熔融石英毛细管柱(35.2 cm×50μm I.D.,有效长度为26.9 cm),运行缓冲液为20 mmol.L-1硼酸溶液(含200 mmol.L-1十二烷基硫酸钠,pH值8.0)-异丙醇-甲醇(体积比70∶20∶10),分离电压为18 kV,检测波长为210 nm,压力自动进样。结果古伦宾和蝙蝠葛任碱的线性范围分别为0.210～1.68 g.L-1和0.026～0.21 g.L-1,线性关系良好。平均加样回收率分别为100.5%和96.1%,RSD分别为3.4%和3.6%(n=6)。应用该法测定10批金果榄药材中古伦宾和蝙蝠葛任碱的含量。结论该方法准确、可靠、环保,适用于金果榄中古伦宾和蝙蝠葛任碱的同时含量测定。

Determination of columbin and menisperine in Tinosporae Radix by micellar electrokinetic capillary chromatography

Objective To develop a micellar electrokinetic capillary chromatographic method for the determination of columbin and menisperine in Tinosporae Radix. Methods Separation was carried out in an uncoated fused-silica capillary (35.2 cm × 50 μm I.D., 26.9 cm effective length) using 20 mmol·L^-1 boric acid (pH 8.0)-isopropanol-methanol（V:V:V =70:20:10）as running buffer at 18 kV. The detection wavelength was set at 210 nm. Results The linear ranges of columbin and menisperine were 0.210-1.68 g·L^-1 and 0.026-0.21 g·L-1, respectively. The average recoveries of columbin and menisperine were 100.5% and 96.1%, and the relative standard deviations (RSDs) were less than 3.6% (n=6). Conclusion The method is simple and economical and can be applied for the determination of columbin and menisperine in Tinosporae Radix.