仙人掌多糖对大鼠局灶性脑缺血的神经保护作用

摘要目的观察仙人掌多糖对大脑中动脉栓塞（MCAO）诱导的大鼠缺血 再灌注损伤的神经保护作用。方法雄性SD大鼠随机分为假手术组、缺血组、0.9%氯化钠溶液组、仙人掌多糖治疗组（200 mg·kg－1·d －1，ip）。采用MCAO法制作大鼠缺血 再灌注损伤模型。分别于大鼠缺血2 h再灌注3，6，8 h后进行神经行为学评分；8 h后2，3，5 三苯基氯化四氮唑（TTC）染色测定脑梗死体积；缺血2 h再灌注22 h后脑组织切片苏木精 伊红（HE）染色显微镜下形态学观察。结果与0.9%氯化钠溶液组相比，再灌注8 h后仙人掌多糖治疗组神经行为学评分平均下降（2.35±0.47）分（P＜0.05），梗死灶体积减少（P＜0.05）；大鼠皮质及海马组织神经细胞丢失、神经胶质增生、核固缩、核深染等形态学均有明显改善（P＜0.05）。结论仙人掌多糖可以缓解大鼠大脑中动脉栓塞症状，具有一定的神经保护作用。

Neuroprotective Effect of Cactus Polysaccharides on Focal Cerebral Ischemia in Rats

Objective To study the neuroprotective effect of cactus polysaccharides(CP) on ischemia/reperfusion(I/R) injury induced by the middle cerebral artery occlusion(MCAO) in rats.Methods The male SD rats were randomly divided into sham-operated,MCAO/R model group,NS-treated group,CP-treated group(200 mg·kg-1·d-1,ip).The MCAO/R model was made by MCAO method.The neurological deficits were detected at 3 h,6 h and 8 h after MCAO for 2 h and reperfusion;The cerebral infarct volume was detected by TTC staining 8 h later,the brain tissue sections of MCAO for 2 h and reperfusion for 22 h were stained by hematoxylin-eosin(H&E) for the morphological observation under microscope.Results Compared to the NS-treated group,the neurological deficit scores of CP-treated group after reperfusion for 8 h was decreased by(2.35±0.47)(P<0.05),in company with the loss of infarct volume(P<0.05).The rat cortical and hippocampal neuronal cell loss,gliosis,nuclear condensation and nuclear deep dyeing were significantly alleviated(P<0.05).Conclusion The CP can attenuate of arterial embolism in rats and ameliorate cerebral ischemia,which possessing a neuroprotective effect.