茶多酚二甲亚砜合剂对铀矿尘人支气管细胞毒性的防护作用

目的探讨铀矿尘所致人支气管细胞BEAS-2B细胞DNA损伤以及茶多酚二甲亚砜合剂对细胞的防护作用。方法 BEAS-2B细胞中加入铀矿尘、铀矿尘+茶多酚、铀矿尘+二甲亚砜、铀矿尘+茶多酚二甲亚砜合剂染毒24 h后,通过检测微核和多核细胞观察染色体损伤,彗星实验及H2AX蛋白磷酸化检测DNA的损伤,多核细胞法检测细胞次黄嘌呤磷酸核糖转移酶(HPRT)突变。结果与正常对照组相比,BEAS-2B细胞经铀矿尘染毒后,细胞的微核率、多核率和拖尾率明显升高,H2AX蛋白磷酸化表达增高,HPRT突变率明显增高(P<0.05),铀矿尘+茶多酚、铀矿尘+二甲亚砜和铀矿尘+茶多酚二甲亚砜合剂保护能有效降低上述变化(P<0.05),其中茶多酚二甲亚砜合剂的保护效果最好。结论铀矿尘对BEAS-2B细胞DNA具有损伤作用,茶多酚二甲亚砜合剂有较强防护作用。

Protection of tea polyphenol and DMSO mixture against uranium dust-induced cytotoxicity in human bronchial epithelial cells

OBJECTIVE To observe the uranium dust-induced DNA damage in BEAS-2B cells and the protective effect of tea polyphenol(TP) and DMSO mixture. METHODS Uranium dust, uranium+TP, uranium+DMSO, uranium+TP+ DMSO were added into BEAS-2B cells for 24 h. Chromosomal damage was observed by micronucleus and multinuclear cells, DNA damage by comets assay, and expression of H2AX phosphorylated proteins and hypoxanthine phosporibosyltransferase (HPRT) gene mutations were assayed by multinucleated cell method. RESULTSCompared with normal control group, the micronucleus rate, multinuclear rate, tailing rate, expression of H2AX protein phosphorylation and HPRT gene mutation rate in BEAS-2B cells were significantly increased (P<0.05) after BEAS-2B cells were exposed to uranium dust. Uranium+TP, uranium+DMSO and uranium+TP+DMSO effectively reduced the changes(P<0.05). Uranium+TP+DMSO mixture had the best protective effect. CONCLUSION Uranium dust can cause DNA damage in BEAS-2B cells. TP and DMSO mixture has a stronger protective effect.