| 大豆皂苷对α-葡萄糖苷酶抑制作用的研究 |
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| 引用本文： | 全吉淑,尹学哲,金明,沈明化.大豆皂苷对α-葡萄糖苷酶抑制作用的研究[J].中药材,2003,26(9):654-656. |
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| 作者姓名： | 全吉淑 尹学哲 金明 沈明化 |
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| 作者单位： | 1. 延边大学医学院,延吉,133000
2. 延边大学医学院附属医院,延吉,133000 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 9960 0 73 ) |
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| 摘 要： | 目的：研究大豆皂苷对α-葡萄糖苷酶(EC3．2．1．20)的抑制作用。方法：以C18反相柱层析和高效液相色谱法从大豆胚轴分离大豆皂苷，比色法测定大豆皂苷单体对α-葡萄糖苷酶的抑制活性。结果：大豆皂苷具有很强的α-葡萄糖苷酶抑制作用，且表现为非竞争性抑制作用。其中B类、E类和DDMP皂苷的抑制作用为最强，其半抑制浓度(IC50)值为10-40μmol／L；A类皂苷的抑制作用则较弱。结论：大豆皂苷是α-葡萄糖苷酶抑制剂，经常食用可能有助于延缓糖尿病患者餐后血糖的持续升高。
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| 关 键 词： | 大豆皂苷 α-葡萄糖苷酶 抑制作用 研究 糖尿病 大鼠 |
| 修稿时间： | 2003年4月14日 |
Study on the Inhibition of alpha-Glucosidase by Soyasaponins |
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| Quan Jishu,Yin Xuezhe,Jin Ming,Shen Minghua.Study on the Inhibition of alpha-Glucosidase by Soyasaponins[J].Jorunal of Chinese Medicinal Materials,2003,26(9):654-656. |
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| Authors: | Quan Jishu Yin Xuezhe Jin Ming Shen Minghua |
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| Institution: | Yanbian University, Yanji 133000. |
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| Abstract: | OBJECTIVE: To study the inhibitory effects of soyasaponins on alpha-glucosidase (EC3.2.1.20). METHODS: Soyasaponins were isolated by ODS column chromatography and high-performance liquid chromatography (HPLC) from hypocotyls of soybean. The inhibitory activities of each component of soyasaponins against alpha-glucosidase were tested by colorimetric method. RESULTS: Soyasaponins showed potent inhibitory activities against alpha-glucosidase. Group B, group E and DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins showed stronger potency, which were non-competitive inhibitors of alpha-glucosidase with IC50 values of 10-40 mumol/L. While group A saponins showed a little lower potency with IC50 values of about 2 mmol/L. CONCLUSION: The results suggest soyasaponins, which exhibit inhibitory effects on alpha-glucosidase, seem physiologically useful for suppressing postprandial hyperglycemia in patients with diabetes mellitus. |
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