同种异体组织工程化软骨修复关节软骨缺损

目的　探讨应用同种异体组织工程化软骨修复软骨缺损的可行性。方法　取新西兰大白兔双膝关节软骨细胞 ,经体外培养扩增 ,与PlruonicF12 7混合 ,植入人为造成的异体兔膝关节软骨缺损。结果　空白对照组和材料对照组只见少许纤维组织修复 ,缺损凹陷 ;实验组 8周后关节软骨缺损区由部分白色透明样软骨组织充填 ,Masson三色染色见胶原分布较均匀 ,软骨陷窝多见 ,未见明显炎症现象。 16周后缺损完全修复 ,缺损表面较光滑 ,部分颜色呈淡兰色 ,软骨陷窝清晰 ,细胞与基质分布均匀 ,未见炎症和退变现象。结论　同种异体组织工程化软骨可用于修复关节软骨缺损。

Repair of Articular Cartilage Defects with Homograft of Tissue-engineering Cartilage

Objective To investigate of the feasibility of using homograft of tissue engineering cartilage for the repair of cartilage defects.Method Chondrocytes obtained from 4 weeks old New Zealand immature rabbits,which were cultured and mixed with Pluronic F127,were transplanted into defedcts of articular cartilage that had been made previously and were 4mm in diameter,extending down to the calcified zone.After the opertion,the rabbits were killed at 4,8and 16 weeks respectively.Results After 8 weeks of the operation,the cartilage were showed in some superficial areas,16 weeks after implantation,the defects were completely fillde with mature cartilage tissue,which integrated smoothly with the preserved articular cartilage in grafted groups at the same time,only a small amount fibrous tissues were appeased in the defect of controlled groups.Conclusion It is a good way to repair articular cartilage defects with homograft of tissue engineering cartilage.