| 高效液相色谱法测定不同生长年限南方红豆杉中紫杉醇的含量 |
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| 引用本文： | 曾志平,王实强. 高效液相色谱法测定不同生长年限南方红豆杉中紫杉醇的含量[J]. 中南药学, 2008, 6(1): 56-57 |
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| 作者姓名： | 曾志平 王实强 |
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| 作者单位： | 1. 中南大学药学院,长沙,410013
2. 湖南省中医药研究院中药研究所,长沙,410013 |
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| 摘 要： | 目的 采用高效液相色谱法测定不同生长年限南方红豆杉中紫杉醇的含量。方法 采用Hypersil BDS C18色谱柱（200nlm×4．6mm，5μm），流动相为甲醇-水（58：42），流速1．0mL·min^-1，检测波长为227nm，柱温为25＇C。结果紫杉醇的线性范围为192～960ng，r=0．9999，平均回收率为97．56％，RSD为1．17％。结论 建立的方法可靠，可用于南方红豆杉中紫杉醇的含量测定。
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| 关 键 词： | 南方红豆杉 紫杉醇 含量测定 高效液相色谱法 |
| 文章编号： | 1672-2981（2008）01-0056-02 |
| 收稿时间： | 2007-05-30 |
| 修稿时间： | 2007-07-24 |
HPLC determination of paclitaxel in Taxus Chinensis var. Mairei with different growth years |
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| ZENG Zhi-ping,WANG Shi-qiang. HPLC determination of paclitaxel in Taxus Chinensis var. Mairei with different growth years[J]. Central South Pharmacy, 2008, 6(1): 56-57 |
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| Authors: | ZENG Zhi-ping WANG Shi-qiang |
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| Affiliation: | ZENG Zhi-ping, WANG Shi-qiang (1. School of Pharmaceutical Sciences, Central South University, Changsha 410013; 2. Hunan Academy of Chinese Traditional Medicine and Mareria Medica , Changsha 410013) |
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| Abstract: | Objective To determine paclitaxel in Taxus Chinensis var. Mairei with different growth years by HPLC. Methods The Hypersil lADS C18 （200 mm× 4.6 mm, 5 μm） column was used. The mobile phase was methanol-water （58：42） and the flow rate was 1.0 mL·min^-1 , the UV detection wavelength was 227 nm, and the column temperature was 25 ℃. Results The linear range of paclitaxel was 192～960 ng （r=0. 999 9）. The average recovery was 97.56% and the RSD was 1.17%. Conclusion The method is reliable and provides helpful evidence for collecting Taxus Chinensis vat. Mairei. |
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| Keywords: | Taxus Chinensis var Mairei paclitaxel determination HPLC |
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