化学去细胞神经同种异体移植后近期运动功能的恢复

目的研究去细胞同种异体神经移植修复大型哺乳类动物粗大和长段周围神经缺损的效果.方法15只犬分成去细胞神经同种异体移植组6只、自体神经移植组6只、新鲜神经同种异体移植组3只.右侧坐骨神经主干造成5.0 cm长缺损,分别以上述3种神经移植物桥接修复.术后6个月进行步态分析.结果同种异体移植组和自体神经移植组功能恢复一般情况相似,正常组、同种异体移植组及自体神经移植组犬距小腿关节角度平均极大值分别为59.0.,58.0.和61.9.,平均极小值分别为16.4.,7.6.和8.4..新鲜神经同种异体移植组无任何恢复;同种异体移植组和自体神经移植组的右后肢运动及步态周期非常相似,距小腿关节跖屈及背伸肌群的肌力均有一定程度的恢复,但不及正常犬.结论化学去细胞神经同种异体移植修复犬坐骨神经长段缺损,在术后6个月近期运动功能恢复与自体神经移植相似.

Recent motor functional recovery after acellular nerve allograft by chemical extraction

OBJECTIVE: To study the effect of acellular nerve allograft on therestoration of the defects in thick and long segment of peripheral nerve in bigmammalia.METHODS: Fifteen canines were grouped into acellular nerve allograftinggroup(ANG group) with 6 canines, autografting group(AG group) with 6canines, and fresh nerve allografting group(FNG group) with 3 canines. A5.0 cm defect was made in the right side of sciatic nerve in all animals andrepaired by above three nerve grafts respectively. A gait analysis was operatedat 6 months after operation.RESULTS: The general functional recoveries in ANG and AG groups weresimilar. The mean maximum value of angle of ankle joint in the canines ofcontrol, ANG, AG groups were 59.0°, 58.0°, and 61.9° respectively and themean minimum values were 16.4°, 7.6° and 8.4° respectively. There was norecovery in FNG group. The hindlimb movement and gait cycle of ANG andAG group were very similar and the myodynamic of flexor and extensormuscle had a certain degrees of recovery but inferior to normal canines.CONCLUSION: There are similar functional recoveries at 6 months aftersurgery in the restoration of long segment defect in sciatic nerve in canines byeither acellular nerve allograft or autograft.