| HPV16L1／L2蛋白单克隆抗体的制备 |
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| 引用本文： | 耿宜萍.HPV16L1／L2蛋白单克隆抗体的制备[J].细胞与分子免疫学杂志,1996,12(1):32-34. |
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| 作者姓名： | 耿宜萍 |
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| 作者单位： | 西安医科大学免疫病理学研究室 |
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| 摘 要： | 用真核表达的ＨＰＶ１６Ｌ１／Ｌ２蛋白作为抗原，经免疫、融合、选择性培养、克隆化等过程，我们建立了两株抗ＨＰＶ１６Ｌ１／Ｌ２蛋白的杂交瘤细胞株（另有数株仍在建株中）。免疫斑点法检测证明，它们产生的抗体，只与ＨＰＶ１６Ｌ１／Ｌ２蛋白起反应，而不与ＨＰＶ１６Ｅ６、Ｅ７蛋白、人血清蛋白和牛血清蛋白起反应。另外，免疫组化试验证明，这种抗体可应用于临床ＨＰＶ１６感染的检验，具有敏感、特异、准确、快捷、经济的优点。
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| 关 键 词： | 人乳头瘤病毒16型（HPV16） L1／L2蛋白 单克隆抗体 |
Preparation of Monoclonal Antibodies against HPV16 L1 /L2 Protein |
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| Liu Yu,etal.Preparation of Monoclonal Antibodies against HPV16 L1 /L2 Protein[J].Journal of Cellular and Molecular Immunology,1996,12(1):32-34. |
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| Authors: | Liu Yu etal |
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| Abstract: | Two hybridoma cell lines secreting McAbs: against HPV16 virus-like particles, which comprises eukaryotic expression system derived L1/L2 capsid proteins, were established. The antibodiesthey secreted only react with HPV16 virus-like Particles while have no reaction with HPV16 E6/E7protein, human serum albumin or bovine serum albumin.It was proved immunohistochemically thatthese antibodies could be sensitively and specifically used in clinical detection of HPV16 infection. |
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| Keywords: | human Papillomavirus type 16(HPV16) virus-like particles monoclonal antibody |
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