| 从EST克隆中筛选肝癌内高表达的基因片段 |
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| 引用本文： | 李宝安 万大方. 从EST克隆中筛选肝癌内高表达的基因片段[J]. 细胞与分子免疫学杂志, 1996, 12(2): 17-19 |
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| 作者姓名： | 李宝安 万大方 |
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| 作者单位： | 第四军医大学病理解剖学教研室，上海市肿瘤研究所 |
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| 摘 要： | 应用Ｎｏｒｔｈｅｒｎ杂交技术，从表达序列标记（Ｅｘｐｒｅｓｓｅｄｓｅｑｕｅｎｃｅｔａｇ，ＥＳＴ）克隆中筛选出一个在肝癌组织内高表达，而在相应癌旁肝及正常肝组织内低表达或不表达的基因片段。ＤＮＡ序列测定表明，该基因片段为一未知新基因的部分序列。此基因片段可作为探针，进一步筛选ｃＤＮＡ文库，以得到新的癌基因的候选基因。
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| 关 键 词： | 肝细胞肝癌；癌基因；表达序列标记 |
Isolation of A Gene Fragment from Clones of Expressed Sequence Tag(EST) Highly Expressed in Hepatocellular Carcinoma |
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| Li Baoan, Liu Yanfang, Wan Dafang, Gu Jianren. Isolation of A Gene Fragment from Clones of Expressed Sequence Tag(EST) Highly Expressed in Hepatocellular Carcinoma[J]. Chinese journal of cellular and molecular immunology, 1996, 12(2): 17-19 |
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| Authors: | Li Baoan Liu Yanfang Wan Dafang Gu Jianren |
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| Abstract: | We applied the technique of Northern blot to isolate from EST (expressed sequencetag) clones a cDNA fragment differentically expressed in normal liver tissue versus hepatocellularcarcinoma. The results showed that F9391 clone expressed at a high level in all the 6 hepatocellular carcinoma samples but at a low level or with no expression in the 6 non cancerous liver tissuesof the same samples and 2 normal liver tissues. Nucleotide sequencing indicated that this clonewas part of a novel gene that had not included in the Gene Database(EMBL 96. 4). This cDNAfragment will be used as a probe to screen cDNA library for obtaining the full lenth cDNA of thisnovel gene. |
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| Keywords: | hepatocellular carcinoma oncogene EST. |
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