| 粒细胞集落刺激因子及其McAb的研究 |
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| 引用本文： | 李小依,王爱霞,郭振泉,郭小清,张月秋. 粒细胞集落刺激因子及其McAb的研究[J]. 中国医学科学院学报, 1991, 0(1) |
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| 作者姓名： | 李小依 王爱霞 郭振泉 郭小清 张月秋 |
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| 作者单位： | 中国医学科学院协和医院，中国医学科学院协和医院，北京大学生物品，中国医学科学院协和医院，协和医院 北京，北京，北京，北京 |
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| 摘 要： | 利用B细胞杂交瘤技术,用rhG-CSF免疫BALB/c小鼠,将其脾细胞与SP2/0骨髓瘤细胞融合,成功地建立了3株能持续分泌特异抗rhG-CSF McAb的杂交瘤细胞。取其中2D_4-B_4杂交瘤细胞进行染色体数目测定,并将2D_4-B_4瘤细胞注入小鼠腹腔,获得抗体阳性腹水,测定提纯抗体IgG亚型、特异性、稳定性和亲和力,证实该McAb可以和hG-CSF及小鼠G-CSF特异结合。为进一步研究hG-CSF和人工合成G-CSF打下良好的基础。
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| 关 键 词： | 粒细胞集落刺激因子 单克隆抗体 杂交瘤细胞 |
Studies on Monoclonal Antibody Against Recombinant Human Granulocyte Colony-stimulating Factor |
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| Li Xiaoyi. Studies on Monoclonal Antibody Against Recombinant Human Granulocyte Colony-stimulating Factor[J]. Acta Academiae Medicinae Sinicae, 1991, 0(1) |
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| Authors: | Li Xiaoyi |
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| Abstract: | Three hybridomas producing monoclonal antibodies (McAb) against recom-binant human granulocyte colony-stimulating factor (rhG-CSF) have been established by fusing mouse myeloma SP 2/0 cells with spleen cells from a BALB/c mouse immunized with rhG-CSF.Ascites was produced from BALB/c mice by injecting substrain 2D4-B4 hybridoma cells intraperitoneally. The antibody was purified either by the caprylic acid-ammonium sulfate method or by euglobulin precipitation. The caprylic acid-ammonium sulfate method was superior to euglobulin precipitation in terms of purification and recovery of IgG. The 2D4-B4 McAb was determined to belong to the IgG3 subclass with a molecular weight of about 172 400 It was proved by Western Blot to react specifically with rhG-CSF. The stability and affinit affinity of the McAb were analyzed as well.The potential clinical and experimental applications of this McAb are discussed. |
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| Keywords: | granulocyte CSF McAb hybridoma cell |
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