宫颈病变中HPV16/18感染与P16^INK4A蛋白表达的关系

目的通过检测宫颈癌及其癌前病变组织中HPVl6／18感染和P16^INK4A蛋白表达的情况，探讨两者之间的关系以及它们在病理诊断中的意义。方法应用荧光定量PCR技术对宫颈组织进行HPV DNA定性检测，采用Envision两步免疫组织化学染色方法检测P16^INK4A蛋白表达。结果268例宫颈组织中HPVl6／18的总检出率为53．73％（144／268），其检出率随CINI级、CINⅡ～Ⅲ级和宫颈鳞癌病变程度的加重而显著增高（P〈0．05）。P16^INK4A“蛋白表达的阳性率在慢性宫颈炎、CINI级、CINⅡ一Ⅲ级和宫颈鳞癌组织中两两比较均有显著性差异（P〈0．05）。HPVl6／18感染与P16^INK4A蛋白的表达呈正相关关系（r8=0．689，P〈0．01）。结论HPV16／18感染可能通过影响P16^INK4A“蛋白的过表达与宫颈癌及其癌前病变的发生发展密切相关，二者联合检测对宫颈癌的筛查和预防具有重要意义。

The Relationship betweenHPV16/18 Infection and the Expression of P16INK4A Protein in Cervical Cancers andPrecursors

Objective To examine the infection with human papillomavirus 16/18 and the expression of P16^INK4A in cervical cancers their precursors, explore the relationship between HPV infection and P16^INK4A protein expression and their significance in pathological diagnosis, Methods All specimens were detected for HPV DNA using techniques of fluorescence quantitative polymerase chain reaction. The expression of P16^INK4A protein were studied by Envision immunohistochemistry. Results The positive rates of HPV16/18 in 268 cases of cervical tissues were calculated to be 53.73 % （144/268）.The more serious the cervical lesions with CIN I , CIN Ⅱ～Ⅲand cervical squamous cell carcinoma were, the more higher the positive rate of HPV16/18 was（P〈 0.05）. The positive rate of P16r^INK4A protein expression was significant in cervical tissues with different degree of cervical lesions（P〈 0.05）. Significant relationship was observed bwteen HPV16/18 infection and P16^INK4A protein expression（r8= 0.689,P〈 0.01）. Conclusion The development of cervical carcinoma and early cervical dysplasia may be associated with an increased risk of HPV16/18 infection and the overexpression of P16^INK4A protein. It is very significant for screening and prevention of cervical cancers to examine HPV infection and the expression of P16^INK4A protein.