营养性肥胖大鼠氧应激水平的改变

目的观察营养性肥胖大鼠氧应激、抗氧化能力的改变。方法20只断乳的SD雄性大鼠(75~80g)随机分两组,每组10只。膳食诱导肥胖组(DIO组)喂饲高脂饲料,对照组喂饲基础饲料,10周后取血及睾周、肾周脂肪组织。用ELISA法检测血浆8-表氧-前列腺素2α(8-epi-PGF2α)、二硫代双硝基苯甲酸法测血浆抗氧化酶GPX(谷胱甘肽过氧化物酶)活性、黄嘌呤氧化酶法测血浆SOD(超氧化物歧化酶)活性,HPLC(高效液相色谱仪)测血浆维生素E并经血脂校正。同时测血浆空腹血糖、胰岛素、三酰甘油(TG)、总胆固醇(TCHL)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)等糖脂代谢指标。结果高脂喂养10周后,DIO组的各项肥胖指标(体重、腹部脂肪/体重、Lee’s指数)均高于对照组(P<0.01);血浆8-epi-PGF2α增高,SOD和GPX活性、经血脂校正后的血浆维生素E水平显著降低(P<0.05)。另有脂代谢紊乱,空腹血糖与胰岛素也显著高于对照组。结论高脂喂养肥胖大鼠模型显示血脂升高并伴有胰岛素抵抗;氧应激水平增高而抗氧化能力下降。

Oxidative stress in a rat model of dietary-induced obesity

OBJECTIVE: To evaluate the levels of antioxidative indexes, lipid metabolism and glycometabolism in high fat diet-induced obese rats. METHODS: A rat model of high fat diet induced obesity was established by feeding weaning male Sprague-Dawley rats with high-fat diet for 10 weeks. plasma 8-epi-prostaglandin-F2 (8-epi-PGF2), alpha-tocopherol, superoxide dismutase activity (SOD), and glutathione peroxidase activity (GSH-Px) were detected. Parameters of lipid metabolism and glycometabolism were also tested by standard methods. RESULTS: After 10 weeks, the high fat diet-induced obesity and lipid metabolism and glycometabolism disorders of obese rats (n = 10) were found. In the comparison with the control groups (n = 10), plasma 8-epi-PGF2 levels of obese rats were more higher, while plasma adjusted alpha-tocopherol (divided by plasma lipids), SOD and GSH-Px activities of obese rats were more lower. CONCLUSION: Obesity induced by high-fat feeding involves increased oxidative stress and decreased antioxidant capacity.