慢性乙型肝炎患者外周血单核细胞中FasL表达 |
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引用本文: | 顾艳英,廖可育.慢性乙型肝炎患者外周血单核细胞中FasL表达[J].中国当代医药,2009,16(11):57-58. |
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作者姓名: | 顾艳英 廖可育 |
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作者单位: | 1. 常德职业技术学院附属一医院,湖北常德,415000 2. 常德职业技术学院,湖北常德,415000 |
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摘 要: | 目的:检测慢性乙型病毒性肝炎患者外周血单核细胞中的FasL的表达,探讨HBV诱导外周血单核细胞凋亡的可能作用机制。方法:PCR检测外周血单核细胞中HBV—DNA,流式细胞术检测凋亡,蛋白印迹检测FasL表达。结果:30例慢性乙型肝炎患者外周血单核细胞中共检出HBV—DNA阳性24例,阴性6例。阳性组凋亡率为(38.14±5.23)%,阴性组凋亡率为(22.37±6.47)%,两者比较,差异有统计学意义(P〈0.05)。阳性组FasL表达明显高于阴性组。结论:慢性乙型肝炎患者外周血单核细胞中FasL高表达可能参与HBV诱导外周血单核细胞凋亡。
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关 键 词: | 慢性乙型肝炎 外周血单核细胞 FasL |
Expression of FasL in peripheral blood mononuclear cells of Chronic hepatitis B |
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Authors: | GU Yanying LIAO Keyu |
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Institution: | GU Yanying,LIAO Keyu (1.Affiliated hospital of ehangde vocational technical college,Changde 415000,China;2.Changde vocational technical college 415000, China) |
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Abstract: | Objective: To detective the expression of FasL in peripheral blood mononuelear cells(PBMC) of Chronic hepatitis B(CHB) to study the mechanism involved in peripheral blood mononuelear cell apoptosis induced by HBV. Methods: HBV DNA in peripheral blood mononuclear cells was deteetived by PCR, FCM for apoptosis and western blot was used for expression of FasL in PBMC. Results: There were 24 CHB Patients with HBV DNA positive and 6 CHB patients negative in peripheral blood mononuelear cell. The apoptosis rate in positive group was (38.14±5.23)%,where negative group was (22.37± 6.47)%.The expression of FasL significantly increased in positive group than negative group.Conclusion: Over-expression of FasL in PBMC of CHB maybe involved in PBMC apoptosis indueed by HBV. |
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Keywords: | FasL |
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