缺氧缺血性脑病新生儿自然杀伤细胞与T细胞亚群的变化及其相关因素

目的了解缺氧缺血性脑病(HIE)新生儿自然杀伤(NK)细胞及T细胞亚群的变化,观察其与各种因素的相关性。方法应用流式细胞仪测定50例HIE和20例正常新生儿血NK细胞、CD3 、CD4 、CD8 细胞及CD4 /CD8 比值。结果HIE患儿NK细胞和T细胞亚群的表达均明显低于正常新生儿(P均<0.05);中、重度HIE患儿NK细胞和CD3 、CD4 细胞及CD4 /CD8 比值明显均低于轻度HIE(P均<0.05),而不同分度HIE的CD8 细胞则无统计学差异(P均>0.05);NK细胞、CD3 、CD4 及CD4 /CD8 与HIE病情分度均呈负相关(P均<0.05)。NK细胞表达与胎龄呈正相关;CD3 细胞与羊水污染呈负相关(P<0.05);CD4 细胞与羊水污染呈负相关,与出生体质量和Apgar评分呈正相关(P<0.05);CD4 /CD8 比值与分娩方式存在线性相关(P<0.05)。结论缺氧缺血可导致HIE患儿NK细胞活性抑制及T淋巴细胞亚群紊乱,某些因素对细胞免疫功能有一定影响。

Changes of Natural Killer Cells and T Lymphocyte Subpopulations in Neonates with Hypoxic-Ischemic Encephalopathy and Its Correlation Factors

Objective To investigate the changes and correlative factors of natural killer(NK)cells and T lymphocyte subpopulations levels in neonates with hypoxic-ischemic encephalopathy(HIE).Methods Peripheral blood samples were collected from 50 neonates with HIE.The percentages of NK cell(CD3~-CD_(16)~ CD_(56)~ ) and T lymphocyte subpopulations including CD3~ ,CD4~ ,CD8~ and CD4~ /CD8~ ratio were determined by Flow Cytometr(FCM).Meanwhile,20 healthy neonates were served as controls.Results (Le)-vels of NK cell,CD3~ ,CD4~ ,CD8~ and CD4~ /CD8~ ratio in neonates with HIE were significantly lower than those in control group(all P<0.05).Levels of NK cell,CD3~ ,CD4~ and CD4~ /CD8~ ratio were significantly lower in neonates with(moderate) to severe HIE than those in mild HIE(all P<0.05).There was no significant difference of percentage of CD8~ among the stages of HIE(all P>0.05).Spearman correlation analysis showed that there were negative correlation between the NK cell,CD3~ ,CD4~ ,CD4~ /CD8~ ratio and stages of HIE(all P<0.05).No relationship was found between CD8~ and stages of HIE(all P>0.05). Linearregression analysis showed that there was a positive correlation between NK cell and gestationalages((P<0.05)),and there was a negative correlation between CD3~ and amniotic fluid(P<0.05).Positive correlations were found between CD4~ and birth weight,as well as Apgar score,and a negative correlation was found between CD4~ and amniotic fluid(P<0.05).CD4~ /CD8~ ratio was correlated with the mode of delivery(P<(0.05)).Conclusions Hypoxic-ischemic can lead to NK cell depressing and T lymphocyte subpopulations disordering.Some factors have effects on immune function of neonates with HIE.