| 脂多糖对人牙髓细胞增殖和碱性磷酸酶活性的影响 |
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| 引用本文： | 王凤明,胡涛,周学东.脂多糖对人牙髓细胞增殖和碱性磷酸酶活性的影响[J].上海口腔医学,2005,14(1):77-80. |
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| 作者姓名： | 王凤明 胡涛 周学东 |
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| 作者单位： | 四川大学华西口腔医学院,牙髓病研究室,四川,成都,610041;四川大学华西口腔医学院,牙髓病研究室,四川,成都,610041;四川大学华西口腔医学院,牙髓病研究室,四川,成都,610041 |
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| 基金项目： | 四川省科技厅重点科技攻关项目(03SG022-002) |
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| 摘 要： | 目的:了解脂多糖(LPS)对人牙髓细胞的增殖和碱性磷酸酶活性的影响。方法:Ⅰ型胶原酶消化组织块法体外培养牙髓细胞,检测细胞表面LPS受体CD14(mCD14);流式细胞术观察不同浓度LPS(0.1μg/ml、1μg/ml和10μg/ml)对牙髓细胞细胞周期的影响,测定不同浓度LPS对牙髓细胞碱性磷酸酶活性的影响,采用χ2检验以及单因素方差分析对实验数据分别进行统计学分析。结果:人牙髓细胞不表达mCD14,LPS在浓度为0.1μg/ml、1μg/ml和10μg/ml时可以抑制牙髓细胞增殖(P<0.01)及碱性磷酸酶活性(P<0.05)。结论:LPS具有抑制牙髓细胞增殖及牙髓细胞分化的作用。
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| 关 键 词： | 牙髓细胞 脂多糖 细胞增殖 碱性磷酸酶 |
| 文章编号： | 1006-7248(2005)01-0077-04 |
| 修稿时间： | 2004年8月24日 |
Proliferation and ALPase activity of human dental pulp cells in response to lipopolysaccharide in vitro |
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| WANG Feng-ming,HU Tao,ZHOU Xue-dong.Proliferation and ALPase activity of human dental pulp cells in response to lipopolysaccharide in vitro[J].Shanghai Journal of Stomatology,2005,14(1):77-80. |
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| Authors: | WANG Feng-ming HU Tao ZHOU Xue-dong |
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| Institution: | Institute of Endodontology, West China College of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province, China. |
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| Abstract: | PURPOSE: To explore the cause of orbital fracture associated with diplopia by summarizing 68 cases, in order to offer reference for clinical treatment. METHODS: 68 cases of orbital fracture associated with diplopia were examined by maxillofacial surgeon and oculist, by analyzing the CT and X-ray films of whom to make clear the reasons of diplopia. RESULTS: Of the 68 cases, male was more than female, vertical diplopia was dominant, and generally with the changes of the orbital structure. CONCLUSION: The main cause of diplopia is for the changes of orbital structures. The doctors must take it seriously and give the patients timely treatment. |
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| Keywords: | Dental pulp cell Lipopolysaccharide Cell proliferation Alkaline phosphatase |
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