弱精子症、少弱精子症患者血清、精浆和精子锌含量分析

目的:检测弱精子症和少弱精子症患者血清、精浆和精子锌的含量,分析锌含量的变化与精子密度和精子运动之间的关系。方法:按照WHO《人类精液及精子-宫颈粘液相互作用实验室检验手册》第四版的标准进行精液质量分析,随机筛选出90例弱精子症、60例少弱精子症患者以及20例精液质量正常的生育者作为研究对象,利用原子吸收光谱法检测其血清、精浆、精子的锌含量并进行统计学分析。结果:3组间血清锌含量没有显著差异;弱精子症、少弱精子症患者精浆锌含量均显著低于正常生育者(P<0.05);少弱精子症患者精子锌含量显著高于弱精子症患者和正常生育者(P<0.01)。结论:弱精子症、少弱精子症患者精子的发生及运动功能下降可能与精浆锌含量的低下呈正相关;但过高的精子锌含量与精子的发生和运动功能的关系尚不十分明了,有待进一步研究。

Zinc Content Analysis in Serum, Seminal Plasma and Spermatozoa of Asthenozoospermic and Oligoasthenozoospermic Patients

Objective: To detect the zinc content in serum,seminal plasma and spermatozoa of asthenozoospermic and oligoasthenozoospermic patients,and analyse the relationship between zinc changes and sperm density as well as sperm motility.Methods: Sperm quality analysis was made according to the WHO standard of Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction(4~(th)edition),and then 90 asthenozoospermic patients,60 oligoasthenozoospermic patients and 20 fertile men with normal sperm quality were screened out as the objects to be researched.The zinc content in their blood,seminal plasma and spermatozoa was detected by flame atomic absorption spectroscopy.And then a statistical analysis was made on the test results.Results: The blood zinc content among the 3 groups had no significant difference;the seminal plasma zinc of asthenozoospermic and oligoasthenozoospermic patients were significantly lower than that of fertile men(P<(0.05));the spermatozoa zinc content of oligoasthenozoospermic patients was significantly higher than that of asthenozoospermic patients and fertile men(P<(0.01)),respectively.Conclusion: There may be a positive relationship between the feebleness of sperm production and motility of asthenozoospermic and oligoasthenozoospermic patients and the lower content of seminal plasma zinc;however,the relationship between the higher spermatozoa zinc content and the function of sperm production and sperm motility is still not clear,on which a further study should be made.