重症肌无力患者细胞免疫水平的检测及临床意义

目的 :探讨周围血中 T细胞亚群、NK细胞、细胞膜白细胞介素 - 2受体、可溶性白细胞介素 - 2受体的阳性细胞与 MG发病及临床疗效的关系。方法 :1.应用流式细胞仪检测外周围血 m IL- 2 R(CD2 5)阳性细胞、NK(CD5 6 )和T细胞亚群 (CD3 、CD4、CD8)的相对计数。 2 .用富氏双抗体夹心 EL ISA方法检测血清 s IL- 2 R。结果 :1.MG患者血清s IL- 2 R水平明显高于健康对照组 ,激素治疗后较治疗前明显下降 ;2 .NK和 CD4阳性细胞在治疗前均明显高于正常 ,治疗后明显下降 ;3.CD3 阳性细胞在激素治疗前后无明显改变 ;4.m IL- 2 R(CD2 5)阳性细胞数无明显改变。结论 :1.血清中的 s IL- 2 R,NK细胞的测定可对该病进行动态观察 ;2 .CD4增高 ,CD8下降提示免疫功能紊乱 ;3.m IL- 2 R阳性细胞百分率不能直接反映 MG的免疫功能紊乱。

Detection of cell-mediated immunity and clinical significance in patients with myasthenia gravis

Objective: To investigate if T-cell subsets, NK cell mIL-2R, sIL-2R were related to pathogenesis and therapeutic efficacy of MG. Methods: 1. CD 25 . CD26 CD 3 CD 4 and CD 8 from the peripheral blood were tested by flowcytometre 2. Seral sIL-2R was detected with ELISA. Results: The levels of seral sIL-2R, NK cell and CD 4 cell were higher in MG patients than that of controls before treatment and decreased dignificantly after corticosteroids treatment. There were no significant changes of mIL-2R and CD 3. Conclusion: 1. Dynamic detection of sIL-2R, NK cell is important for clinical evalution of the effect 2. CD 4 increase and CD 8 decrease indicated the disturbance of immune function 3. The percentage of mIL-2R positive cell didn't show the disturbance of immune function.