葡多酚对胸腺细胞凋亡和线粒体膜电位的影响

目的　观察葡多酚 (GPC)对氧化损伤引发的胸腺细胞凋亡和线粒体膜电位的影响。方法　在体外培养的胸腺细胞中加入不同浓度的GPC和培养液中浓度为 12 5 μmol L的H2 O2 ,共同培养 2h ,采用流式细胞术检测细胞凋亡和线粒体膜电位水平。结果　氧化损伤组细胞凋亡率为 (2 9 5 3± 3 8) % ,线粒体膜电位为 2 7 2 4± 2 2 (D值 ) ,5 0mg LGPC保护组则分别为 (8 6 1± 1 2 ) %和 87 5 5± 4 7(D值 ) ,两组间的差异均有显著性 (P <0 0 5 )。结论　GPC可有效抑制氧自由基引发的胸腺细胞凋亡和线粒体膜电位的下降 ,对胸腺细胞的氧化损伤有良好防护作用

Effect of grape procyanidins on the apoptosis and mitochondrial transmembrane potential of thymus cells

Objective Investigating the effect of grape procyanidins(GPC) on the apoptosis and mitochondrial transmembrane potential of thymus cells damaged by H 2O 2. Methods Thymus cells of mouse were cultured in vitro. The cells were pretreated by GPC and then damaged by 125μmol/L H 2O 2 for two hours. The levels of apoptosis and mitochondrial transmembrane potential were analysed by flow cytometry. Results Apoptosis rate and the level of mitochondrial transmembrane potential of positive group were (29 53±3 8)% and 27 24±2 2(D value) respectively. After pretreatment of 50mg/L GPC ,the levels of apoptosis and mitochondrial transmembrane potential were (8 61±1 2)%and 87 55±4 7(D value). The differences between the two groups were significant ( P <0 05). Conclusion GPC can inhibit apoptosis and decrease of mitochondrial transmembrane potential of thymus cells damaged by oxygen free radical.