散发性结直肠癌患者血RASSF2和sFRP1启动子区甲基化检测

目的检测散发性结直肠癌患者血清RASSF2和sFRP1启动子区甲基化情况．从而为散发性结直肠癌的早期筛查提供参考。方法使用甲基化特异性PCR检测59例散发性直结肠癌患者和59例健康对照血清sFRP1和RASSF2基因启动子区甲基化的情况，并分析其与结直肠癌临床病理特征的关系。结果59例结直肠癌患者中RASSF2和sFRP1甲基化者分别为16例（27．1％）和18例（30．5％）；而59例健康对照无一例发现RASSF2或sFRP1基因甲基化，差异有统计学意义（均P〈0．01）。29例（49．2％）结直肠癌患者有至少1个基因甲基化，其甲基化率明显高于RASSF2和sFRP1单-基因甲基化率（均P〈0．05）。结直肠癌患者血清RASSF2和sFRP1基因甲基化率与结直肠癌临床病理特征均无明显关系（均P〉0．05）。结论血清RASSF2和sFRP1基因启动子区甲基化水平在结直肠癌组织异常升高，联合两基因的血清甲基化检测可为散发性结直肠癌的早期筛查提供参考。

Detection of RASSF2 and sFRP1 promoter region methylation in sporadic colorectal cancer patients

Objective To investigate the methylation in promtor region of RASSF2 and sFRP1 in sporadic colorectal cancer patients in order to provide screening method for early colorectal cancer. Methods The methylation in promoter region of RASSF2 and sFRP1 in serum samples of 59 sporadic colorectal cancer patients and 59 healthy volunteers was detected by methylation specific PCR. The association between clinicopathological features of sporadic colorectal cancer patients and methylation in promoter region of RASSF2 and sFRP1 was analyzed. Results The methylation rates of RASSF2 and sFRP1 gene in serum of 59 sporadic colorectal cancer patients were 27.1% and 30.5%, significantly higher than those in healthy volunteers （0%, both P〈O.01 ）. The methylation of RASSF2 or sFRP1 occurred in 29（49.2%） patients, which was significantly higer than the methylation rate of single gene（P〈0.05）. No association was found between methylation ratio of RASSF2 and sFRP1 and clinicopathological features in sporadic colorectal cancer patients. Conclusions Methylation in promoter region of RASSF2 and sFRP1 is often detected in serum of colorectal cancer patients. The combination detection of methylation for the two genes may provide information for early screening of colorectal cancer.