人胎盘贴壁细胞定向诱导神经样细胞

目的 探讨胎盘组织培养贴壁细胞的分离培养以及定向诱导神经细胞分化的生物潜能，从而研究胎盘贴壁细胞与神经发育的相互关系。方法 用胰酶消化分离培养人胎盘组织中的贴壁细胞，并应用抗体Nestin、MAP2、MBP、CD133、CD34等进行免疫细胞化学染色，用2-巯基乙醇和反式维甲酸进行体外向神经细胞诱导分化。结果 分离培养出胎盘组织贴壁细胞，是一异质性细胞群体。培养的贴壁细胞表达CD133，不表达CD34，同时表达Nestin和MAP2。经定向神经诱导后的人胎盘贴壁细胞出现神经样细胞的形态，强表达Nestin和MAP2，弱表达MBP和GFAP。结论 人胎盘贴壁细胞经体外定向诱导可分化为神经样细胞，其具有间充质干细胞（MSC）性质，胎盘组织是一个MSC的重要来源。

Neural cell induction with human placenta derived adherent cells

Objective The expression of hematopoietic stem cell and neural markers on placenta adherent cells and their association were investigated, the potential of neural differentiation of placenta adherent cells may demonstrate the relationship between placenta adherent cells and hematogenesis and neurogenesis. Methods Isolated adherent cells in human placenta tissue were stained immunocytochemically with Nestin, MAP_2, MBP, CD133, CD34 antibody to detect protein production and induced neural differentiation with β-mercaptoethanol and retinoic acid. Results Isolated placenta adherent cells confirmed to be heterogenous expressed CD133, Nestin, MAP_2 weakly and CD34 none. While induced cells showed neural like morphology, strong positive for Nestin, MAP_2 and weak positive for MBP and GFAP in immunocytochemical staining. Conclusion Human placenta adherent ceils can be induced into neural like cells in vitro with histological characteristics of mesenchymal stem cell(MSC) ,it provides a new source for clinical application of hMSCs.