| 大鼠脊髓损伤继发骨质疏松的实验研究 |
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| 引用本文： | 李颖智 杨有赓 于艳波 刘占军 徐春华 崔丽. 大鼠脊髓损伤继发骨质疏松的实验研究[J]. 实验动物与比较医学, 2004, 24(3): 134-141 |
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| 作者姓名： | 李颖智 杨有赓 于艳波 刘占军 徐春华 崔丽 |
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| 作者单位： | 1. 上海市农业科学院畜牧兽医研究所,上海,201106;上海市农业遗传育种重点实验室动物遗传工程研究室,上海,201106
2. 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
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| 基金项目： | 德国BMBF:CHN99／310上海市科学技术委员会基金资助课题(No.014909005)资助。 |
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| 摘 要： | 猪的体细胞核移植研究在生物医学领域很有意义。本试验对克隆技术中关键步骤之一的去核方法作了深入研究。结果表明,由本实验室改进的Willadsen去核法--二步挤压去核法无论在去核操作成功率(73.9％)上,还是在去核效率(81.2％)上都显著地高于McGrath-Solter去核法(42.5％、67.4％,P<0.05)。还发现,卵母细胞成熟培养36 h后去核组去核效率(89.1％)显著地高于成熟培养44 h后去核组(55.8％,P<0.05)。
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| 关 键 词： | 猪 卵母细胞 核移植 去核 体细胞克隆 |
| 文章编号： | 1004-8448(2004)03-0134-04 |
| 修稿时间： | 2004-05-31 |
An Experimental Study of Secondary Osteoporosis of Spinal Cord Injured Rats |
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| LI Ying-zhi,YANG You-geng,YU Yang-bo,LIU Zhang-jun,XU Chun-hu,CUI Li. An Experimental Study of Secondary Osteoporosis of Spinal Cord Injured Rats[J]. Laboratory Animal and Comparative Medicine, 2004, 24(3): 134-141 |
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| Authors: | LI Ying-zhi YANG You-geng YU Yang-bo LIU Zhang-jun XU Chun-hu CUI Li |
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| Affiliation: | LI Ying-zhi 1,YANG You-geng 1,YU Yang-bo 2,LIU Zhang-jun 3,XU Chun-hua 1,CUI Li 4 |
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| Abstract: | The study on porcine somatic nuclear transfer is of the great meaning in the field of biomedicine. This experiment was conducted to study enucleated method, which is one of the key techniques for animal cloning. The results show that the modified Willadsen's method for enucleation, called two-step-squeezing method, is significantly better than McGrath-Solter's method in the enucleated rates (73.9%, 42.5%, P<0.05) and the rates (81.2%, 67.4%, P<0.05) of absolutely removing nuclei matter. It was also found that the rates (89.1%) of absolutely removing nuclei matter for 36h-matured-in-vitro oocytes is significantly higher than that for 44h group (55.8%, P<0.05). |
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| Keywords: | Pig Oocytes Enucleation Nuclear transfer |
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