HPLC法测定万氏牛黄清心微丸中盐酸小檗碱和黄芩苷的含量

目的测定万氏牛黄清心胶囊中盐酸小檗碱和黄芩苷的含量。方法采用高效液相色谱法 ,以盐酸小檗碱和黄芩苷为对照品 ,采用Shim PackCLC ODSC18柱 ,乙腈 0 1mol·L-1磷酸二氢钾溶液 (V∶V =3∶7)为流动相 ,流速为 1 0mL·min-1,在波长 2 80nm检测。结果盐酸小檗碱在6 0 4～ 48 3 2mg·L-1内 ,质量浓度与峰面积具有良好的线性关系 ,线性回归方程为A =2 2 5 6×1 0 4ρ-8 72 0× 1 0 3 ,r =0 9998;平均回收率为 1 0 2 0 % (RSD =1 73 % ,n =9)。黄芩苷在 1 1 88～71 2 8mg·L-1内 ,质量浓度与峰面积具有良好的线性关系 ,线性回归方程为A =7 2 1 0× 1 0 3 ρ -1 674× 1 0 2 ,r=0 9997;平均回收率为 99 4% (RSD =2 3 4% ,n=9)。结论测定方法可用于万氏牛黄清心胶囊的质量控制

Determination of the contents of berberine hydrochloride and baicalin in Wanshi Niuhuang Qingxin Pellets by HPLC

Objective To determine the contents of berberine hydrochloride and baicalin in Wanshi Niuhuang Qingxin Pellets.Method A high-performance liquid chromatographic method was developed for the determination.A Shim-Pack CLC-ODS C 18 column( 150 mm× 6 mm, 5 μm)was used,with the mobile phase of acetonitrile:KH 2PO 4(V∶V= 3∶7),at the detection wavelength of 280 nm.Result The linear range of berberine hydrochloride was 6 04～ 48 32 mg·L -1(r= 0 999 8);the mean recovery was 102 0%(n=9),RSD=1 73%;The linear range of baicalin was 11 88～ 71 28 mg·L -1(r= 0 999 7);the mean recovery was 99 4%(n=9),RSD=2 34%.Conclusion The method can be used in the quality control of Wanshi Niuhuang Qingxin Pellets.