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TLMV5‘非编码区基因的克隆与序列分析
引用本文:周伯平 陈心春. TLMV5‘非编码区基因的克隆与序列分析[J]. 中华实验和临床病毒学杂志, 2000, 14(3): 244-246
作者姓名:周伯平 陈心春
作者单位:深圳市肝病研究所,深圳
摘    要:目的 调查TTV阴性的非甲 ̄庚型肝炎患者中TTV-like mini virus(TLMV)感染情况,对TLMV5’非编码区(5’NCR)部分基因进行分子克隆与序列分析。方法 采用巢式PCR技术检测53例TTV阴性的非甲-庚肝炎患者血清TLMV DNA,对PCR产物进行克隆、测序和分析。结果 53例病例中TLMV DNA阳性37例(69.8%),对其中8株TLMV基因克隆测序,并与Takahash

关 键 词:TLMV 聚合酶链反应 分子克隆 序列分析
修稿时间:2000-04-30

Molecular cloning andsequencing of 5
ZHOU Bop-ing. Molecular cloning andsequencing of 5[J]. Chinese journal of experimental and clinical virology, 2000, 14(3): 244-246
Authors:ZHOU Bop-ing
Affiliation:Shenzhen Institute of Hepatology, Shenzhen 518020, China.
Abstract:OBJECTIVE: To investigate the TTV- like mini virus (TLMV) infection in Chinese patients with non A-G hepatitis and to analyse sequence of 5' non-coding region (5'NCR)of TLMV. METHODS: TLMV DNA was detected by nested - PCR from 53 serum samples of non A-G hepatitis patients without TTV infection. PCR products were cloned and sequenced. RESULTS: TLMV DNA were positive in 37 of 53 patients (69.8%). The sequence of 5' NCR of 8 TLMV isolates was compared with that reported by Takahashi (GenBank Accession No. ab 026930-026931) and showed nucleotide identity between 64% - 83%. CONCLUSIONS: The results of this study indicated that TLMV infection is common in TTV negative patients with non A-G hepatitis. The 5' NCR sequence of TLMV showed a great degree of diversity. The pathogenicity of TLMV and its relationship to non A-G hepatitis need to be confirmed and further study is required.
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