CD147和MMP-2在大肠癌组织中的表达及血清水平变化的意义

目的: 研究CD147和MMP-2在大肠癌组织中的表达和大肠癌患者血清中的水平变化,探讨其与大肠癌侵袭和转移的相关性,及二者间的相互联系。 方法: 应用免疫组织化学(SABC)法结合图像分析软件检测44例大肠癌、癌旁和正常对照组织中CD147和MMP-2的表达及半定量测定。应用酶联免疫吸附实验(ELISA)测定44例大肠癌患者血清中CD147和MMP-2的水平,并设36例健康对照。 结果: 大肠癌组织CD147和MMP-2的表达显著高于癌旁和正常对照组织,其表达与肿瘤分型、分化程度未见相关,而与转移密切相关。大肠癌患者血清CD147和MMP-2水平显著高于健康对照组,且伴转移组血清中CD147水平显著高于无转移组;低分化组中MMP-2水平显著高于高、中分化组。两者在组织定量和血清水平呈正相关。 结论: 大肠癌组织、血清中CD147高表达及高含量在一定程度上可以反映大肠癌发展程度及转移状态,其促MMP-2激活和表达增高的作用可能是其中一个重要因素。

Significance of the Expression and Serum Levels of CD147 and MMP-2 in Patients with Colorectal Cancer

Objective: To study the expression and serum levels of CD147 and MMP-2 protein in patients with colorectal cancer, to investigate the correlation between CD147 and MMP-2, and to explore the relationship of CD147 and MMP-2 with the invasion and metastasis of malignant tumor. Methods: Immunochemical staining was performed to detect the expression of CD147 and MMP-2 protein in colorectal cancer tissues, adjacent tissues, and normal controls. Semi-determination was performed by image analysis software. ELISA wa...